Western Blot Substrates and Substrate Kits

Western Blot Substrates and Substrate Kits
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MilliporeSigmaâ„¢ Immobilon Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
Product Type | HRP Substrate |
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For Use With (Application) | Dot/Slot Blot |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
Substrate | Horseradish Peroxidase |
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Sensitivity | Mid-femtogram |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 24 hr. |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
MilliporeSigmaâ„¢ Immobilonâ„¢ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price
Substrate | Horseradish Peroxidase |
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Content And Storage | 2°C to 8°C |
Shelf Life | 9 Months |
For Use With (Application) | Dot/Slot Blot |
Cytiva Amershamâ„¢ ECL Selectâ„¢ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciencesâ„¢ Amershamâ„¢ ECL Selectâ„¢ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Thermo Scientificâ„¢ SuperSignalâ„¢ West Atto Ultimate Sensitivity Substrate
SuperSignalâ„¢ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Shipping Condition | Wet Ice |
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Substrate | Horseradish Peroxidase |
Sensitivity | Low-femtogram to high-attogram |
Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ Pierceâ„¢ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Substrate | Horseradish Peroxidase |
---|---|
Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 1 to 2 hr. |
Product Line | Pierceâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ Pierceâ„¢ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Sensitivity | Low picogram to mid-femtogram |
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Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierceâ„¢ |
Detection Method | Chemiluminescence, Fluorescence |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Pico PLUS and SuperSignalâ„¢ West Femto Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots
Thermo Scientificâ„¢ 1-Stepâ„¢ Chloronaphthol Substrate Solution
1-Stepâ„¢ Chloronaphthol Substrate Solution
MilliporeSigmaâ„¢ Luminata Crescendo ELISA HRP Substrate
Used on immunoblots as chemiluminescent substrate
Content And Storage | 2°C to 8°C |
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Thermo Scientificâ„¢ SuperSignalâ„¢ West Femto and SuperSignalâ„¢ West Pico PLUS Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots
Thermo Scientificâ„¢ SuperSignalâ„¢ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto
Contains SuperSignalâ„¢ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignalâ„¢ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.
Thermo Scientificâ„¢ 1-Stepâ„¢ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.