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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
2

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

3

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

4

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
5

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

6

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)  DFS Item

Optimized, easy-to-use blocker and primary antibody diluent system

7

Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)  DFS Item

All buffers are made with high-purity reagents and are strictly quality controlled

8

Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)  DFS Item

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels

9

Invitrogen™ Incubation Tray, 10 x 14 cm  DFS Item

Enables convenient and even staining of E-PAGE™ blots

10

Applied Biosystems™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer  DFS Item

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

11

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

12
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Invitrogen™ iBind™ Flex Western Starter Kit  DFS Item

Allows better antigen-antibody interaction and solutions flow between the iBind™ Card and membrane for maximum interaction between protein and antibody

14

Invitrogen™ ZOOM™ CHAPS  DFS Item

For use with ZOOM IEF Fractionator

15

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)