Protein Method Blocking Buffers

Thermo Scientific™ SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Invitrogen™ I-Block™ Protein-Based Blocking Reagent  

Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.

Invitrogen™ eBioscience™ IHC/ICC Blocking Buffer - High Protein

Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC/ICC Blocking Buffer, High Protein is recommended for tissues and antibodies with high background staining, nuclear antigens, and FFPE tissue.

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 20X), with 2% Tween 20

MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets

Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C

Thermo Scientific™ Fish Serum Blocking Buffer

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.

MilliporeSigma™ Calbiochem™ BLOT-QuickBlocker™ Reagent

Prevents nonspecific proteins from binding to blots.

MilliporeSigma™ HAT Assay Buffer, 5X

For use in the HAT Assay.

Thermo Scientific™ StartingBlock™ Blocking Buffer

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific Chemicals Tween 20 washing buffer, 1% in PBS (10X)

Thermo Scientific Chemicals Tween 20 washing buffer, 0.5% in PBS (10X)

TBS with Tween™ (TBST), 20X Solution, Molecular Biology Grade, Ultrapure

Used in Western blotting protocols

Thermo Scientific™ SuperBlock™ Blocking Buffer, blotting

Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.

Thermo Scientific™ Pierce™ Fast Blocking Buffer

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with Thermo Scientific™ Pierce Fast Blocking Buffer, a ready-to-use blocker solution.