Search results for "Enzo Life Sciences"
Enzo BIOMOL Green (1x250ml)
A simple and convenient method for colorimetric phosphate quantitation. Applications: FUNC. Colorimetric detection, HTS. Contents: Biomol Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4C; can be stored frozen without deleterious effects. Phosphate Standard (Prod. No. BML-KI102) (1ml; 800m phosphate in distilled water) Storage: room temperature. Long-term at 4C; can be stored frozen without deleterious effects.
Enzo PROTEOSTAT Aggresome detection kit (1x25tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT.. Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent. Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Enzo Goat anti-rat IgG, polyclonal antibody (HRP conjugate) (0.5 ml)
Host: Goat. Immunogen: Purified rat IgG (whole molecule). Species Reactivity: Rat. Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Applications: ELISA, ICC, IHC, WB. Recommended dilutions: ELISA (1:5,000-1:100,000). Immunocytochemistry (1:500-1:5,000). Immunohistochemistry(1:500-1:5,000). Western Blot (1:10,000-1:200,000, ECL). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Immunoaffinity purified. Formulation: Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol. Handling: Avoid freeze/thaw cycles.
Enzo Poly(ADP-ribose) monoclonal antibody (10H) (100 l)
Clone: 10H. Host: Mouse. Isotype: IgG3. Immunogen: Purified poly(ADP-ribose). Species Reactivity: Human, Mouse, Rat, Drosophila. Specificity: Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme. Applications: Flow Cytometry, ICC, IHC (PS), WB. Recommended dilutions: Immunocytochemistry (5-20g/ml). Immunohistochemistry (paraffin sections; dilution buffer: 5% milk (non fat dried milk) in PBS to a final. Concentration of 5-20g/ml). Western Blot (incubate 2.5g/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk)). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein A-affinity purified from supernatant. Formulation: Liquid. In 50mM HEPES, pH 7.4,containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide. Handling: Avoid freeze/thaw cycles.
Enzo Ubiquitin monoclonal antibody (P4D1) (100 g)
Clone: P4D1. Host: Mouse. Isotype: IgG1. Immunogen: Denatured bovine ubiquitin. UniProt: P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52). Source: Purified from hybridoma tissue culture supernatant. Species Reactivity: Species independent. Specificity: Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin. Applications: IHC, IP, WB. Recommended dilutions: Western Blot (1:1,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein G affinity purified form cell culture media. Formulation: Liquid. In PBS containing 0.01% sodium azide. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20C.
Enzo Fis1 polyclonal antibody (100 g)
Host: Rabbit. Isotype: IgG. Immunogen: Synthetic peptide surrounding aa 143 of human Fis1. UniProt: Q9Y3D6. Species Reactivity: Human, Mouse, Rat. Applications: WB. Recommended dilutions: Western Blot (1-4 g/ml). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Affinity purified. Formulation: Liquid. In PBS containig 0.5% BSA, 0.01% thimerosal and 30% glycerol.
Enzo ATF6 monoclonal antibody (70B1413.1) (100 g)
Clone: 70B1413.1. Host: Mouse. Isotype: IgG1. Immunogen: Partialhuman ATF6 (activating transcription factor 6) containing aa 1-273. UniProt: P18850. Species Reactivity: Human, Mouse, Rat, Rabbit, Hamster. Applications: ICC, IHC (PS), IP, WB, ChIP. Recommended dilutions: Western Blot (1-5g/ml). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein G-affinity purified. Formulation: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20C.
Enzo p62 (human) polyclonal antibody (100 l)
Host: Rabbit. Immunogen: Synthetic peptide corresponding to aa 387-436 human p62. UniProt: Q13501. Source: Purified from rabbit serum. Species Reactivity: Human. Applications: ELISA, IF, IHC, WB. Recommended dilutions: Immunohistochemistry (1:1,000). Western Blot (1:1,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Peptide affinity purified. Formulation: Liquid. In PBS containing 0.09% sodium azide. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20C.
Enzo HSP 70 HSP 72 monoclonal antibody (C92F3A-5) (50 g)
Clone: C92F3A-5. Host: Mouse. Isotype: IgG1. Immunogen: Native human Hsp70 protein. UniProt: P0DMV8 (HSPA1A), P0DMV9 (HSPA1B). Source: Purified from ascites. Species Reactivity: Human, Mouse, Rat, Xenopus, Teal, Sheep, Rabbit, Porcine, Monkey, Hamster, Guinea pig, Gerbil, Fish, Drosophila, Dog, Chicken, C. elegans, Bovine, Beluga. Applications: ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB, Electron microscopy. Recommended dilutions: Immunohistochemistry (1:50). Western Blot (1:1,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein G affinity purified. Formulation: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. Handling: Avoid freeze/thaw cycles.
Enzo LC3B monoclonal antibody (5F10) (100 g)
Clone: 5F10. Host: Mouse. Isotype: IgG1. Immunogen: Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin UniProt: Q9GZQ8. Species Reactivity: Human, Mouse, Rat, Hamster, Dog. Specificity: Recognizes both forms of endogenous LC3B. Applications: ICC, WB. Purity: Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography. Formulation: Lyophilized from 1ml PBS containing PEG, sucrose and 0.09% sodium azide.
Enzo Calcitonin gene related peptide polyclonal antibody (100 l)
Host: Rabbit. Immunogen: Synthetic peptide corresponding to a portion of rat -calcitonin gene-related peptide (CGRP). UniProt: P01256. Source: Whole rabbit serum. Species Reactivity: Human, Mouse, Rat. Applications: IHC. Formulation: Liquid. Antiserum containing 0.1% sodium azide.
Enzo Bassoon monoclonal antibody (SAP7F407) (50 g)
Clone: SAP7F407. Host: Mouse. Isotype: IgG2a. Immunogen: Recombinant rat Bassoon. UniProt: O88778. Source: Purified from hybridoma tissue culture supernatant. Species Reactivity: Human, Mouse, Rat, Dog, Bovine. Applications: ICC, IF, IHC, IP, WB. Recommended dilutions: Immunocytochemistry (1:400). Immunoprecipitation (2.5g). Western Blot (1:500 - 1:1,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein G affinity purified. Formulation: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. Handling: Avoid freeze/thaw cycles.
Enzo TNF-alpha (soluble) (mouse), (recombinant) (50 g)
Alternative name: Tumor necrosis factor alpha, TNFSF 2. MW: ~20kDa (SDS-PAGE). Concentration: 1mg/ml after reconstitution. Endotoxin content: E. coli. The extracellular domain of mouse TNF- (tumor necrosis factor-) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG-tag. Specificity: Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF- shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-. Use/Stability: Stable for at least 6 months after receipt when stored at -20C. UniProt: P06804. Application: ELISA. Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20C. Long Term Storage: -20C
Enzo Proteasome 20S beta1i subunit monoclonal antibody (LMP2 - 13) (100 l)
Clone: LMP2-13. Host: Mouse. Isotype: IgG1. Immunogen: Recombinant human 1i protein. UniProt: P28065 (human), P28077 (rat). Source: Purified from ascites. Species Reactivity: Human, Rat. Specificity: Recognizes the 1i subunit of proteasome 20S. Applications: IHC, WB. Purity: Protein G-affinity purified ascites. Formulation: Liquid. In PBS containing 10mM sodium azide.
Enzo Mouse IgG1 isotype control, monoclonal antibody (MOPC-21) (PE conjugate) (50 g)
Clone: MOPC-21. Host: Mouse. Isotype: IgG1. Source: Purified from ascites. Species Reactivity: Species independent. Applications: Flow Cytometry. Recommended dilutions: Flow Cytometry (10g/ml). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Purity: Protein G-affinity purified. Formulation: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. Handling: Avoid freeze/thaw cycles. Protect from light.