missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing
  1. Home
  2. Products
  3. Cell Culture
  4. Cell Culture Media
  5. Mamallian Cell Culture Media
  6. Cell Culture Media
  7. Gibco™ DMEM, High Glucose, Pyruvate
  8. 11995065

Gibco™ DMEM, High Glucose, Pyruvate DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.

Manufacturer:  Gibco™ LS11995065

 View more versions of this product

Catalog No. 11995065


 

Disclaimers:

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.


Requires : Supplementation

Specifications

Description

Media, Cell Culture; Gibco; DMEM; High Glucose; Pyruvate; Mammalian cell culture; Liquid; 500mL

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

See Product Certificates

Description & Specifications

Specifications

Mammalian Cell Culture
Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, HEPES, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
DMEM media
Storage: 2-8° C. Protect from light
HEPES
Liquid
Sustainable packaging
500mL
High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate