
Reverse Transcription Products




Applied Biosystemsâ„¢ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Shipping Condition | Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Quantity | Each |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
GC-Rich PCR Performance | High |
---|---|
Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Quantity | Each |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
Shipping Condition | Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript II |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ One-Step RT-PCR System with Platinumâ„¢ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogenâ„¢ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
GC-Rich PCR Performance | High |
---|---|
Format | 1-step RT-PCR Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 13 kb |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Hot Start | Built-in Hot Start |
Optimal Reaction Temperature | 50°C to 65°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
PCR Method | 1-step RT-PCR |
Multiplex Capability | Yes |
Detection Method | Gel Electrophoresis |
Invitrogenâ„¢ Second Strand cDNA Synthesis Kit
Invitrogenâ„¢ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Reaction Format | Separate Components |
---|---|
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
For Use With (Application) | Cloning |
Invitrogenâ„¢ SuperScriptâ„¢ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
GC-Rich PCR Performance | High |
---|---|
Format | Master Mix |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Concentration | 4X |
Technique | Template Switching Reverse Transcription |
Starting Material | Cells or RNA |
Thermo Scientificâ„¢ Verso cDNA Synthesis Kit
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.
Shipping Condition | Wet or Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | Verso |
Size (Final Product) | Up to 11 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Invitrogenâ„¢ SuperScriptâ„¢ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 30 to 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ III First-Strand Synthesis System
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogenâ„¢ SuperScriptâ„¢ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Invitrogenâ„¢ SuperScriptâ„¢ III CellsDirectâ„¢ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
GC-Rich PCR Performance | High |
---|---|
Format | Kit |
Reverse Transcriptase | SuperScript III |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | Cells |
For Use With (Application) | Real Time PCR (qPCR) |
Invitrogenâ„¢ SuperScriptâ„¢ VILOâ„¢ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |