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Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #2 includes: Anti-Mouse CD4 FITC, CD25 PE and CD16/32, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a Isotype Control APC, staining buffer, fixation concentrate.
Includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage (IL-17A, IL-17F, IL-21 and IL-22).
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Panel and Staining Buffers are designed for flow cytometry-based identity testing of regulatory T-cells (Tregs).
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.