Flow Cytometry Compensation, Counting and Calibration
Invitrogenâ„¢ eBioscienceâ„¢ Foxp3 / Transcription Factor Staining Buffer Set
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
Invitrogenâ„¢ eBioscienceâ„¢ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Invitrogenâ„¢ eBioscienceâ„¢ Fixation/Permeabilization Concentrate
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogenâ„¢ eBioscienceâ„¢ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogenâ„¢ eBioscienceâ„¢ Fixation/Permeabilization Diluent
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogenâ„¢ UltraComp eBeadsâ„¢ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Molecular Probesâ„¢ CountBrightâ„¢ Absolute Counting Beads, for flow cytometry
CountBrightâ„¢ Absolute Counting Beads, for flow cytometry
Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 μm
Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 μm
Molecular Probesâ„¢ Cell Sorting Set-up Beads (for green-yellow lasers)
Cell Sorting Set-up Beads (for green-yellow lasers)
Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm
Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm
Invitrogenâ„¢ eBioscienceâ„¢ Mouse Cytokine Positive Control Cells
Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.
Invitrogenâ„¢ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.
Invitrogenâ„¢ eBioscienceâ„¢ Mouse Hematopoietic Lineage Biotin Panel
Contains 5 biotinylated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their cell surface antigen expression.
Invitrogenâ„¢ eBioscienceâ„¢ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Invitrogenâ„¢ eBioscienceâ„¢ Mouse Regulatory T Cell Staining Kit #2
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscienceâ„¢ Mouse Regulatory T Cell Staining Kit #2 includes: Anti-Mouse CD4 FITC, CD25 PE and CD16/32, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a Isotype Control APC, staining buffer, fixation concentrate.