Protein Mass Spectrometry Sample Prep

Protein Mass Spectrometry Sample Prep
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Thermo Scientificâ„¢ Pierceâ„¢ LTQ Velos ESI Positive Ion Calibration Solution
Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.
Thermo Scientificâ„¢ Pierceâ„¢ FlexMixâ„¢ Calibration Solution
Thermo Scientificâ„¢ Pierceâ„¢ FlexMixâ„¢ Calibration Solution is a mixture of 16 highly pure, ionizable components designed for both positive and negative ionization calibration using Orbitrap Tribrid Series 3.1 Instrument Control Software.
Thermo Scientificâ„¢ SureQuantâ„¢ IP-MS Sample Preparation Kits
Thermo Scientific SureQuant IP-MS Sample Preparation Kits provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis.
Content And Storage | Store trypsin at 4°C. Store trypsin storage solution at -20°C. |
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Workflow Step | Sample prep enrichment |
For Use With (Equipment) | Mass spectrometer |
Quantity | 20 Reactions |
For Use With (Application) | MS-compatible immunoprecipitation |
Starting Material | Cells,Tissue |
Detection Method | Mass Spectrometry |
Product Line | SureQuantâ„¢ |
Thermo Scientificâ„¢ EasyPepâ„¢ MS Sample Prep Kits
Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
Starting Material | Protein Samples |
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Detection Method | Mass Spectrometry |
Product Line | EasyPepâ„¢ |
Thermo Scientificâ„¢ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
Workflow Step | Protein Digestion |
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For Use With (Equipment) | Mass Spectrometer |
Grade | MS |
Detection Method | Mass Spectrometry |
Product Line | Pierceâ„¢ |
Thermo Scientificâ„¢ Pierceâ„¢ C18 Spin Tips & Columns
Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.
Content And Storage | Store at room temperature. |
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Workflow Step | Sample Clean Up |
Final Product Type | Peptides |
For Use With (Equipment) | Microcentrifuge, Mass Spectrometer |
Starting Material | Peptides,Protease-digested Protein |
Detection Method | Mass Spectrometry |
Product Line | Pierceâ„¢ |
Thermo Scientificâ„¢ Pierceâ„¢ Negative Ion Calibration Solution
Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.
Thermo Scientificâ„¢ Pierceâ„¢ Triple Quadrupole Calibration Solution, extended mass range
Obtain both positive and negative ionization calibration of Thermo Scientificâ„¢ Triple Stage Quadrupole instruments using the Thermo Scientificâ„¢ Pierceâ„¢ Triple Quadrupole Calibration Solution, Extended Mass Range, which is a mixture of 14 highly pure, ionizable components (mass range: 69 m/z to 2800m/z).
Thermo Scientificâ„¢ EasyPepâ„¢ Magnetic MS Sample Preparation Kits and Proteomics Magnetic Clean-up Beads
EasyPep Magnetic MS Sample Preparation Kits and Proteomics Magnetic Clean-up Beads contain MS-compatible magnetic beads specifically designed to tolerate harsh MS sample prep conditions to improve sample quality and reproducibility for both manual and automated sample preparation methods.
Form | Liquid |
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Starting Material | Protein Samples |
Detection Method | Mass Spectrometry |
Thermo Scientificâ„¢ Pierceâ„¢ Peptide Retention Time Calibration Mixture
15 synthetic heavy peptides mixed in equimolar ratio to test and calibrate reverse-phase liquid chromatography (LC) and mass spectrometry (LC-MS) equipment.
Thermo Scientificâ„¢ Pierceâ„¢ HeLa Protein Digest Standard
Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
Form | Solid |
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Final Product Type | Peptides |
Product Type | Protein Digest Standard |
For Use With (Equipment) | Mass Spectrometer |
Reagent Type | Standard |
Starting Material | Peptides |
Detection Method | Mass Spectrometry |
Product Line | Pierceâ„¢ |
Thermo Scientificâ„¢ 96-Well Plates for Pierceâ„¢ Quantitative Fluorometric Peptide Assay
Measure total peptide concentration by a mix-and-read fluorescent microplate method that provides improved sensitivity, reproducibility, and ease using the Thermo Scientificâ„¢ Pierceâ„¢ Quantitative Fluorescent Peptide Assay.
Thermo Scientificâ„¢ Pierceâ„¢ Quantitative Peptide Assays & Standards
Pierce colorimetric or fluorescent quantitative peptide assays and standards are easy-to-use microplate assays designed specifically to improve the sensitivity and reproducibility of peptide quantitation for use with mass spectrometry analysis.
Workflow Step | In-Process Testing |
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For Use With (Equipment) | Microplate Reader |
Starting Material | Peptides,Protease-digested Protein |
Product Line | Pierceâ„¢ |
Thermo Scientificâ„¢ Pierceâ„¢ Albumin Serum Depletion Kits
Optimize serum sample prep by depleting unwanted albumin and IgG serum proteins for protein-profiling methods using quality depletion kits.
Final Product Type | Protein |
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Starting Material | Plasma,Serum |
Product Line | Pierceâ„¢ |
Thermo Scientificâ„¢ Pierceâ„¢ High pH Reversed-Phase Peptide Fractionation Kit
Increase protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientificâ„¢ Pierceâ„¢ High pH Reversed-Phase Peptide Fractionation Kit.