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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ ElectroMAX™ DH12S Cells  DFS Item

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production of single-stranded (ss)DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries.

2

Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli  DFS Item

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

3

Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli  DFS Item

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

4

MilliporeSigma™ Annexin V-PE Apoptosis Detection Kit

Identifies phosphatidylserine (PS) that translocates from cytoplasmic face of plasma membrane to cell surface during apoptosis.

5

MilliporeSigma™ Novagen™ Tuner™ DE3 pLysS Competent Cells

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
6

Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

Phage T1 - Résistante (tonA) No
Shipping condition (Condition d'expédition) Dry Ice
Clonage de l’ADN méthylé No
Type de produit Chemically Competent Cells
Résistance aux antibiotiques bactérienne Yes (Chloramphenicol)
Contient l’épisome F' No
Espèces E. coli (B)
Compatibilité à haut débit Low
Améliore la stabilité de l’ARN Yes (pLysS)
Promoteur T7
Protéines toxiques No
Format Tube
Sélection bleu/blanc No
Niveau d’efficacité de la transformation Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
Réduit la recombinaison No
Améliore la qualité des plasmatiques No
Améliore la stabilité des protéines Yes (lon, ompT)
Préparation de l’ADN non méthylé Yes (dcm)
Gamme de produit One Shot
7

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli  DFS Item

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

8

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

9

Invitrogen™ TOP10F' Electrocomp™ Kit  DFS Item

TOP10F' Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F' episome.

10

Invitrogen™ MultiShot™ FlexPlate Mach1™ T1R Competent Cells  DFS Item

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

11

Invitrogen™ Tali™ Calibration Beads  DFS Item

Align and calibrate Tali™ Image-Based Cytometer every three months

12

MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Contenu et stockage ≤-70°C
13

MilliporeSigma™ Novagen™ RosettaBlue™ (DE3)pLysS Competent Cells

RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Efficacité Guaranteed efficiency: >1 x 106 cfu/μg
Contenu et stockage ≤-70°C
14

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

15

Invitrogen™ One Shot™ INVαF' Chemically Competent E. coli  DFS Item

One Shot INVαF' Chemically Competent E. coli are designed to stably replicate high-copy number plasmids.