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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli  DFS Item

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

2

MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells

NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.

Efficacité Guaranteed efficiency: >1 x 109 cfu/μg
Contenu et stockage ≤-70°C
3

Invitrogen™ TOP10 Electrocomp™ Kit  DFS Item

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

4

MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
5

Invitrogen™ MAX Efficiency™ DH5α-T1R Competent Cells  DFS Item

MAX Efficiency DH5α T1R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5.

6

Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells  DFS Item

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency.

7

Invitrogen™ MultiShot™ FlexPlate Mach1™ T1R Competent Cells  DFS Item

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

8

Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells  DFS Item

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

9

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Résistance aux antibiotiques des bactéries Yes (Streptamycin, Tetracycline)
Clonage d’ADN méthylé Yes (mcrA)
Type de produit Chemically Competent Cells
Résistant au phage T1 (tonA) No
Espèces E. coli (K12)
Clonage d’ADN instable Not suitable for cloning unstable DNA
Conditions d’expédition Dry Ice
Compatibilité à haut débit Low
Format Tube
Sélection bleue / blanche Yes (lacZΔM15)
Niveau d’efficacité de la transformation High Efficiency (>1 x 109 cfu/μg)
Gamme de produits One Shot
Contient l’épisome F Yes
Réduit la recombinaison Yes (recA1)
Améliore la qualité des plasmides Yes (endA1)
Préparation de l’ADN non méthylé No
Accélérateur Trc, Tac, Lac
10

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

11

Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells  DFS Item

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.

12

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli  DFS Item

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

13

Invitrogen™ MultiShot™ Stbl3™ Chemically Competent E. coli Cells, 96-well plate

MultiShot Stbl3 Chemically Competent E. coli Cells support cloning of unstable vectors with direct repeats. Automation-compatible 96-well format ensures high-throughput use while the Stbl3 strain minimizes recombination and maintains complex constructs.

14

Invitrogen™ One Shot™ BL21(DE3)pLysE Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysE Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

15

MilliporeSigma™ Novagen™ NovaBlue Singles™ Competent Cells

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

Efficacité Guaranteed efficiency: >1.5 x 108 cfu/μg
Contenu et stockage ≤-70°C