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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Méthode de détection | Colorimetric |
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À utiliser avec (équipement) | Spectrophotometer, Microplate Reader |
Type de produit | Protein Quantitation Assay |
Doser | BCA Assay |
Gamme de produits | Pierce™ |
À utiliser avec (application) | Solution-based Detection, Absorbance |
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
For isolation of RNA from cell extracts | CAS: 50-01-1 | CH6ClN3
Couleur | White |
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Synonyme | guanidine hydrochloride |
Nom chimique ou matériau | Guanidine Hydrochloride |
Pureté | ≥99.5% |
CAS | 50-01-1 |
Point de fusion | 178°C |
Merck Index | 15, 4597 |
Plage de pourcentage du dosage | ≥99.5% |
Forme physique | Solid |
Poids de la formule | 95.53 |
Température de stockage | Store at Room Temperature |
Formule moléculaire | CH6ClN3 |
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
MWCO | 7.0 kDa |
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Gamme de produits | Zeba™ |
Cible de purification | Buffer Exchange, Protein |
Type de colonne | Size-exclusion, Proprietary Resin |
Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Clear-to-purple (A750nm), 2-component, folin phenol- and copper-based reagent system to measure total protein concentration vs. protein standard.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Entretoise | Mid-length |
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Perméabilité cellulaire | Cell-Impermeant |
Type de produit | Sulfo-NHS-LC-Biotin |
Solubilité | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Gamme de produits | EZ-Link™ |
Réactivité chimique | Amine |
Marqueur ou colorant | Biotin |
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Plage de dosage | 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL |
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Méthode de détection | Colorimetric |
Type | Endotoxin Quantitation Kit |
Format | Kit |
Cible | Endotoxins |
Doser | End-point Chromogenic Assay |
Sensibilité du dosage | 0.01 EU/mL |
Gamme de produits | Pierce™ |
Conditions d’expédition | Wet Ice |
Contenu et stockage | • Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt. |
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Format | Liquid |
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Type de produit | Extraction Buffer |
Contenu et stockage | Upon receipt store at 4°C. |
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Description | EDC |
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Méthode d’étiquetage | Chemical Labeling |
Perméabilité cellulaire | No |
Cleavable | No |
Type de produit | Crosslinker |
Longueur du bras espaceur | 0.0 Å |
Réactivité chimique | Amine-Carboxyl |
Conditions d’expédition | Wet Ice |
Forme | Powder |
Contenu et stockage | Upon receipt store desiccated at -20°C. |
Pégylé | No |
Entretoise | Short (<10 Å) |
Poids moléculaire | 191.7 |
Format | Standard, Single-use, Premium-grade |
Type d’agent de réticulation | Heterobifunctional |
Solubilité | Water |
Hydrosoluble | Yes |
Groupement de réactifs | Carbodiimide |
The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.
MWCO | 40 kDa |
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Jetable | Single-use |
Gamme de produits | Zeba™ |
Cible de purification | Buffer Exchange, Protein |
Type de colonne | Size-exclusion, Proprietary Resin |
Remove salts and contaminants, and maximize sample recovery using this semi-permeable membrane with a 3,500-Dalton protein MW cut-off (MWCO) cassette that is easier to use than traditional dialysis tubing.
MWCO | 3.5 kDa |
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Format | Cassette |
Gamme de produits | Slide-A-Lyzer™ |
Membrane | Cellulose Membrane, Dialysis |
Cible de purification | Buffer Exchange, Protein |
Contenu et stockage | Store at room temperature |