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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

2

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

3

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

4

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

5

Thermo Scientific™ pTZ19R DNA

Perform DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system with this double-stranded closed circular high copy phagemid DNA.

6

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

7

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

8

Thermo Scientific™ pUC57 DNA

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

9

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells  DFS Item

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

10

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells  DFS Item

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

11

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells  DFS Item

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

12

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix  DFS Item

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Type de contrôle Positive Control
Type de produit HiFi Master Mix
Gamme de produits GENEART
Nombre de fragments Up to 6 fragments
Étape du flux de travail DNA Assembly
Conditions d’expédition Ambient, Wet Ice, Dry Ice
Méthode de clonage Seamless cloning
À utiliser avec (application) Seamless Cloning & Genetic Assembly
Contenu et stockage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
13

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix  DFS Item

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Type de contrôle Positive Control
Type de produit Master Mix
Gamme de produits GENEART
Nombre de fragments Up to 15 fragments
Étape du flux de travail DNA Assembly
Conditions d’expédition Dry Ice
Méthode de clonage Seamless cloning
À utiliser avec (application) Seamless Cloning & Genetic Assembly
Contenu et stockage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
14

Invitrogen™ TrueTag™ Donor DNA Kit, YFP  DFS Item

The Invitrogen TrueTag Donor DNA Kit, YFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

15

Invitrogen™ TrueTag™ Donor DNA Kit, DDK  DFS Item

The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.