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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (220)
DNA Synthesis Reagents and Columns (107)
PCR Cloning Kits (104)
DNA Cloning and Mapping and Mutagenesis (83)
Plasmid DNA (11)

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1

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli  DFS Item

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

2

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
3

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

4

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

5

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Type de cellule Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
6

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit  DFS Item

Provides a 5 minute, one-step cloning strategy. Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR4-TOPO Vector, One Shot™ TOP10 Chemically Competent E. coli and PureLink Plasmid Prep Kit enables direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.

7

Applied Biosystems™ Control DNA (from CEPH Individual 1347-02)  DFS Item

The control DNA can be used with linkage mapping sets MD10, HD5, and LD20.

8

Applied Biosystems™ GeneScan™ 1200 LIZ™ dye Size Standard  DFS Item

The GeneScan™ 1200 LIZ™ Size Standard is a fifth dye (LIZ™) labeled high density size standard for the reproducible sizing of fragment analysis samples.

9

Invitrogen™ Collibri™ Library Amplification Master Mix with Primer Mix  DFS Item

Invitrogen™ Platinum SuperFi Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries.

Heure du test Standard
Fidélité (par rapport à Taq) ≥100
Format de réaction SuperMix or Master Mix
Type de produit Library Amplification Master Mix
Performances de PCR riches en GC High
Gamme de produits Collibri
Hot Start Built-In Hot Start
En surplomb Blunt
Type d’échantillon DNA,RNA
Conditions d’expédition Dry Ice
Forme Liquid
À utiliser avec (application) Sequencing
10

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli  DFS Item

Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Souche bactérienne ou de levures INVαF ́
Format Kit
Type de produit Cloning Kit
Gamme de produits One Shot
Type de cellule Chemically Competent E. coli
Accélérateur T7
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
11

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Souche bactérienne ou de levures TOP10F ́
Format Kit
Type de produit Cloning Kit
Vecteur pCR2.1
Gamme de produits One Shot
Type de cellule Chemically Competent E. coli
Accélérateur T7
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
12

Invitrogen™ RNase T1 (1 U/μL)  DFS Item

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

13

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli  DFS Item

Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Gamme de produits One Shot
Type de cellule Chemically Competent E. coli
Accélérateur T7
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
14

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Type de cellule Chemically Competent
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
15

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.