Standard PCR Reagents and Kits
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Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2
Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
| Polymérase | AmpliTaq DNA Polymerase |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Format de réaction | Kit |
| Type de produit | PCR Buffer |
| Concentration | 10X |
| À utiliser avec (application) | Standard PCR |
Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with or without protocol
The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.
| GC-Rich PCR Performance | Medium |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Fast PCR Master Mix |
| Nbre de réactions | 250 Reactions |
| En surplomb | 3'-A |
| Concentration | 2X |
| Polymérase | AmpliTaq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Volume | 0.1 mL or 0.2 mL |
| Hot Start | Built-In Hot Start |
| Taille (produit final) | 2 kb or less |
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)
Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Standard PCR |
| Polymérase | Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Stand-alone enzyme |
| Activité exonucléasique | 5' - 3' |
| Hot Start | No |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer
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The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.
Applied Biosystems™ AmpliTaq Gold™ 360 Buffer Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.
Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
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The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
| Fidélité (par rapport à Taq) | 1X |
|---|---|
| Format | Stand-alone enzyme |
| Format de réaction | Separate Components |
| Activité exonucléasique | 5' - 3' |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits
Premixed 2X hot start PCR components for convenience and reproducibility
| Type de produit | Hot start master mix kit |
|---|---|
| Contenu et stockage | Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations |
Applied Biosystems™ Fast MicroSEQ™ 500 16S rDNA PCR Kit
The Fast MicroSEQ™ 500 16S rDNA PCR kit utilizes new Fast technology and provides bacterial identifications from colony to results in as little as 5 hours using the Veriti™ 96-well Thermal Cycler.
Applied Biosystems™ CLA IdentiFiler™ Plus PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Applied Biosystems™ Fast MicroSEQ™ D2 Fungal rDNA PCR Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is the PCR component of the FAST MicroSEQ Fungal Identification System, with enough reagents for 50 PCR amplifications of fungal DNA unknowns.
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymérase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Hot Start | No |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
| Polymérase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | PCR Master Mix |
| Hot Start | No |
| En surplomb | 3'-A |
| Concentration | 2X |
| Matériau de départ | DNA |
| À utiliser avec (application) | Standard PCR |
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
| Méthode de détection | Primer-probe |
|---|---|
| Vitesse de réaction | Fast or Standard |
| Type d’échantillon | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Méthode PCR | qPCR,Standard PCR |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Standard PCR |
| Polymérase | Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Stand-alone enzyme |
| Activité exonucléasique | 5' - 3' |
| Hot Start | No |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |