Standard PCR Reagents and Kits
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Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with or without protocol
The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.
| GC-Rich PCR Performance | Medium |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Fast PCR Master Mix |
| Nbre de réactions | 250 Reactions |
| En surplomb | 3'-A |
| Concentration | 2X |
| Polymérase | AmpliTaq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Volume | 0.1 mL or 0.2 mL |
| Hot Start | Built-In Hot Start |
| Taille (produit final) | 2 kb or less |
Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
| Polymérase | Taq DNA Polymerase |
|---|---|
| Type de produit | DNA Polymerase |
| Concentration | 5 U/μL |
| À utiliser avec (application) | Standard PCR |
Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA)
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| Pureté ou qualité | LD (Low DNA) |
| Nbre de réactions | 200 Reactions |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Standard PCR |
| Polymérase | AmpliTaq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Stand-alone enzyme |
| Activité exonucléasique | 5' - 3' |
| Hot Start | No |
| Quantité | 250 units |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits
Premixed 2X hot start PCR components for convenience and reproducibility
| Type de produit | Hot start master mix kit |
|---|---|
| Contenu et stockage | Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations |
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
| Méthode de détection | Primer-probe |
|---|---|
| Vitesse de réaction | Fast or Standard |
| Type d’échantillon | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Standard PCR |
| Polymérase | Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Stand-alone enzyme |
| Activité exonucléasique | 5' - 3' |
| Hot Start | No |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ MicroSEQ™ Full Gene 16S rDNA PCR Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The MicroSEQ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria.
Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl2
Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Format | Kit |
| Type de produit | 10X PCR Gold Buffer |
| Concentration | 10X |
| À utiliser avec (application) | Standard PCR |
Applied Biosystems™ Fast MicroSEQ™ D2 Fungal rDNA PCR Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is the PCR component of the FAST MicroSEQ Fungal Identification System, with enough reagents for 50 PCR amplifications of fungal DNA unknowns.
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Méthode PCR | qPCR,Standard PCR |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Standard PCR |
| Polymérase | Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Stand-alone enzyme |
| Activité exonucléasique | 5' - 3' |
| Hot Start | No |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.
Applied Biosystems™ Fast MicroSEQ™ 500 16S rDNA PCR Kit
The Fast MicroSEQ™ 500 16S rDNA PCR kit utilizes new Fast technology and provides bacterial identifications from colony to results in as little as 5 hours using the Veriti™ 96-well Thermal Cycler.
Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2
Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
| Polymérase | AmpliTaq DNA Polymerase |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Format de réaction | Kit |
| Type de produit | PCR Buffer |
| Concentration | 10X |
| À utiliser avec (application) | Standard PCR |
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
| Méthode de détection | Primer-probe |
|---|---|
| Vitesse de réaction | Fast or Standard |
| Type d’échantillon | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
| Polymérase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | Taq DNA Polymerase (Recombinant) |
| Hot Start | No |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Standard PCR |