missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. High Fidelity PCR Reagents and Kits
  7. Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Numéro de catalogue. 12358010
Encompass
Change view
Click to view available options
No. of Reactions:
100 Reactions
5 x 500 Reactions
500 Reactions
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. No. of Reactions
12358010 100 Reactions
12358050 500 Reactions
12358250 5 x 500 Reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Numéro de catalogue. 12358010 Fournisseur Invitrogen™ Code fournisseur 12358010
Il en reste null

Description

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Features

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets of high GC content
  • Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
  • Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).

Spécifications

Content And Storage • 2X Platinum SuperFi PCR Master Mix (2 x 1.25 mL)
• SuperFi GC Enhancer (1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –5°C to –30°C.
GC-Rich PCR Performance High
Polymerase Platinum SuperFi DNA Polymerase
Reaction Speed Fast
Quantity 100 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 300X
Hot Start Built-In Hot Start
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Size (Final Product) 20 kb or less
Afficher plus de résultats Afficher moins de résultats

For Research Use Only. Not for use in diagnostic procedures.