HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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TSKgel™ SuperH3000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ SuperH3000 Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers with molecular weights less than 60,000 Da.

Pricing and Availability

MilliporeSigma™ Supelco™ SUPELCOGEL™ H, 6% Crosslinked HPLC Column, 9 μm Particle Size

Used for the analysis of saccharides or organic acids.

Pricing and Availability
Specifications

Particle Size	9 μm
Stationary Phase	Sulfonated Polystyrene
Phase	Ion Exclusion
Matrix	Sulfonated Polystyrene/Divinylbenzene Phase/Spherical Particle Platform
Crosslinking	0.06
Technique	Crosslinked HPLC
Product Line	SUPELCOGEL

Thermo Scientific™ Hypercarb™ HPLC Column, 5μm

Thermo Scientific™ Hypercarb™ Columns give you 100% porous graphitic carbon for extended separation capabilities

Pricing and Availability
Specifications

Pore Size	250 Å
Particle Size	5 μm
Carbon Load	100%
Phase	Normal and Reversed Phase
Column Type	Mixed Mode
pH	1 to 14
Surface Area	120 m²/g
USP Type	L109
Column Format	Analytical Column

MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Column, 3.4 μm Particle Size (Dia. 1-4.6 mm)

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core™ particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.

Pricing and Availability
Specifications

Particle Size	3.4 μm
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Matrix	C18 (octadecyl) Phase/Spherical Silica Particle Platform
Endcapped	Yes
pH	1 to 9
Material	Stainless Steel
Technique	Fused-Core HPLC
Surface Area	̃15 m2/g
Product Line	BIOshell

MilliporeSigma™ Supelco™ BIOshell™ A160 Peptide C18 HPLC Column, 160 Å, 2.7 μm Particle Size

BIOshell™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.

Pricing and Availability
Specifications

Pore Size	160 Å
Particle Size	2.7 μm
Carbon Load	4.6%
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Matrix	C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped	No
pH	1 to 8
Material	Stainless Steel Hardware
Technique	Fused-Core HPLC
Surface Area	90 m2/g
Product Line	BIOshell

Thermo Scientific™ POROS™ CaptureSelect™ KappaXL Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ KappaXL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Pore Size	2000 Å
Stationary Phase	Light Chain Kappa affinity ligand, human‐specific
Temperature	2-40°C
Column Type	Affinity
Quantity	1 Column
Material	PEEK
Max. Pressure	2400 psi (170 bar)
Column Format	Analytical Column, Biocompatible PEEK
Particle Size	20 μm
Packing Material	Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Binding Capacity	16.5 mg/ml
pH	1-10
Technique	Bio-LC
Product Line	POROS CaptureSelect

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18-DB HPLC Column, 3 μm Particle Size

SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds.

Pricing and Availability
Specifications

Pore Size	120 Å
Particle Size	3 μm
Stationary Phase	C18 (Octadecyl)
Phase	Reversed Phase
Matrix	C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped	Yes
Surface Area	170 m2/g
Product Line	SUPELCOSIL

Thermo Scientific™ Accucore™ PFP HPLC Columns

Use these solid core particle columns to analyze difficult-to-resolve mixtures including DOA and pharmaceuticals.

Pricing and Availability
Specifications

Pore Size	80 Å
Particle Size	2.6 μm
Carbon Load	5%
Stationary Phase	PFP
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Ultrapure Silica
pH	2 to 8
Surface Area	130 m²/g
Max. Pressure	14500 psi (1000 bar)
Product Line	Accucore
USP Type	L43

MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano HPLC Column, 2.7 μm Particle Size

The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds

Pricing and Availability
Specifications

Pore Size	90 Å
Particle Size	2.7 μm
Stationary Phase	Diisopropyl-Cyanopropylsilane
Phase	Reversed Phase
Matrix	Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform
Endcapped	Yes
Technique	Fused-Core HPLC
Product Line	Ascentis

MilliporeSigma™ Supelco™ Ascentis™ Express C8 UHPLC Column, 2 μm Particle Size

Densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis™ Express 2 μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pricing and Availability
Specifications

Pore Size	90 Å
Particle Size	2 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped	Yes
Technique	Fused-Core UHPLC
Product Line	Ascentis

MilliporeSigma™ Supelco™ apHera™ C18 HPLC Column, 5 μm Particle Size

apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Matrix	C18 (octadecyl) Phase
Product Line	apHera

MilliporeSigma™ Supelco™ Carbotrap™ 202 Thermal Desorption Tubes

Suitable for use with Air Monitoring

Pricing and Availability
Specifications

Type	Thermal Desorption Tube
Matrix	Carbopack™ C Graphitized Carbon Black (GCB), Carbopack™ Graphitized Carbon Black (GCB), Carbotrap™ 202
For Use With (Equipment)	PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer	1/4 in.
Packaging Quantity	10/Pk.
Material	Stainless Steel
Overall Length (English)	3.5 in.
For Use With (Application)	Suitable for Air Monitoring (Compounds: C₅-C₂₀)
Product Line	Carbotrap

MilliporeSigma™ Supelco™ Proteomix™ SCX-NP10 Strong Cation Exchange Column, 10 μm Particle Size, PEEK

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	10 μm
Stationary Phase	Sulfonic Acid
Phase	Cation Exchange
Matrix	Spherical PS-DVB/Sulfonic Acid Phase
pH	2.0 to 12.0
Material	PEEK
Technique	Cation Exchange
Product Line	Proteomix

MilliporeSigma™ Supelco™ CHIRALPAK™ CBH HPLC Column, 5 μm Particle Size

CHIRALPAK™ CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles.

Pricing and Availability
Specifications

Particle Size	5 μm
Stationary Phase	Cellobiohydrolase
Phase	Chiral
Matrix	Cellobiohydrolase Phase
Technique	Chiral HPLC
Product Line	CHIRALPAK

MilliporeSigma™ Supelco™ Astec™ CHIROBIOTIC™ TAG Chiral HPLC Column, 5 μm Particle Size

The removal of three carbohydrate moieties gives CHIROBIOTIC™ TAG complementary selectivity to CHIROBIOTIC™ T.

Pricing and Availability
Specifications

Pore Size	100 Å
Particle Size	5 μm
Stationary Phase	Teicoplanin Aglycone
Phase	Chiral
Matrix	Teicoplanin Aglycone Phase/High-Purity Silica Gel Particle Platform
pH	3.0 to 6.8
Technique	Chiral HPLC
Product Line	Astec CHIROBIOTIC

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