Western Blotting Reagents, Membranes, Supplies

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

GE Healthcare Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. GE Healthcare Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

GE Healthcare Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

GE Healthcare Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  GE Healthcare Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Detect proteins in gels or on membranes via interaction with biotin-labeled bait proteins as primary probes.

Invitrogen™ Novex™ PVDF Pre-cut Blotting Membranes DFS Item

Pre-cut and pre-assembled—ready for protein blotting/transfer

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate: PROMO

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Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

MilliporeSigma™ Immobilon-FL PVDF Membrane

Immobilon-FL PVDF Membrane do not crack or curl, and can be cut without fracturing

Invitrogen™ Novex™ Blotting Roller DFS Item

Compatible with E-PAGE™, Novex Mini Gels, and NuPAGE™ Gels

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

MilliporeSigma™ Immobilon™ PVDF Transfer Membranes: PSQ

For Western blots and protein sequencing

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent PROMO

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Invitrogen™ Novex™ Incubation Tray DFS Item

Enables convenient and even staining of E-PAGE™ blots

MilliporeSigma™ Immobilon ECL Ultra Western HRP Substrate

MilliporeSigma™ Immobilon ECL Ultra Western HRP Substrate excels in all Western and dot/slot/spot blotting applications. Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

GE Healthcare Amersham™ Hybond™-N Membranes

Use for high sensitivity applications in DNA and RNA blotting. GE Healthcare Life Sciences™Amersham™ Hybond™-N Membranes is a strong supported membrane that is inherently hydrophilic and requires no pre-wetting.

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

GE Healthcare Whatman™ Gel Blotting Paper Grade GB003

A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots

Invitrogen™ Invitrolon Novex Invitrolon PVDF/Filter Paper Sandwich DFS Item

Pre-cut and pre-assembled and ready for protein blotting/transfer

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