Western Blotting Reagents, Membranes, Supplies

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Cytiva (Formerly GE Healthcare Life Sciences) Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL Select™ Western Blotting Detection Reagent PROMO

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent PROMO provides highly intense signal output

Thermo Scientific™ SuperSignal™ West HisProbe Kit

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent PROMO

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent PROMO produces high signal intensity and sensitivity

Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Detect proteins in gels or on membranes via interaction with biotin-labeled bait proteins as primary probes.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Polypropylene Microtube Support Racks

Excellent for microbiology work or any work using microtubes

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva (Formerly GE Healthcare Life Sciences) Whatman™ Grade GB005 Blotting Paper

Recommended for applications where fewer layers of blotting paper must still ensure a high capacity. Whatman™ Grade GB005 Blotting Paper ensures the wicking capability and uniformity of capillary action that are important in obtaining clean and even transfers during blotting.

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ start Western Blotting Detection Reagent

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.2 PVDF Western blotting membrane PROMO

0.2 Äμm PVDF blotting membrane for use with standard colorimetric and chemiluminescent detection methods.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

Invitrogen™ Blotting Roller DFS Item

Compatible with E-PAGE™, Novex Mini Gels, and NuPAGE™ Gels

MilliporeSigma™ Immobilon-NC Transfer Membrane

Maximum sensitivity with minimal background, suitable for both chemiluminescent and radioactive detection systems

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