Western Blotting Reagents, Membranes, Supplies

MilliporeSigma™ Immobilon™-P PVDF Membrane

MilliporeSigma™ Immobilon™-P PVDF Membrane

Hydrophobic PVDF Transfer Membrane for western blotting

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ PVDF Transfer Membrane, 0.45 µm, 26.5 cm x 3.75 m

Thermo Scientific™ PVDF Transfer Membrane, 0.45 µm, 26.5 cm x 3.75 m

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

MilliporeSigma™ Immobilon™-FL PVDF Membranes

MilliporeSigma™ Immobilon™-FL PVDF Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

MilliporeSigma™ Immobilon-FL PVDF Membranes

MilliporeSigma™ Immobilon-FL PVDF Membranes

Immobilon-FL PVDF Membrane do not crack or curl, and can be cut without fracturing

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Hoefer™ Blotting Paper

Hoefer™ Blotting Paper

Cellulose papers precut for convenience

Thermo Scientific™ Owl™ Blotting Paper

Thermo Scientific™ Owl™ Blotting Paper

Absorb adequate amounts of buffer for semi-dry electroblotting without the loss of mechanical stability with this high-quality blotting paper.

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

GVS PVDF-Plus™ Transfer Membrane

GVS PVDF-Plus™ Transfer Membrane

Improves detection limits for more senstive Western blots.

Cytiva Whatman™ Grade GB003 Blotting Paper

Cytiva Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls

Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.

Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels) <img src=

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels) DFS Item

Pre-cut and pre-assembled and ready for protein blotting/transfer

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Thermo Scientific™ Pierce™ DAB Substrate Kit

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

GVS Charged Nylon Membranes

GVS Charged Nylon Membranes

Do not require prewetting or vacuum baking

Thermo Scientific™ Low-Fluorescence PVDF Transfer Membranes, 0.2 μm 7 x 8.4 cm

Thermo Scientific™ Low-Fluorescence PVDF Transfer Membranes, 0.2 μm 7 x 8.4 cm

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

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