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Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Catalog No. PI21050
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500 mL
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Catalog No. Quantity
PI21050 500 mL
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Catalog No. PI21050 Supplier Thermo Scientific™ Supplier No. LSG21050

Description

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.

Highlights:

  • Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method

  • Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively

  • Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF

  • Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots

  • Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)

  • Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins

  • Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature

Includes:

Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL

Specifications

Product Type Western Blot Enhancer
Detection Method Colorimetric, Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Quantity 500 mL
Content And Storage Upon receipt store kit at room temperature.

Frequently Asked Questions (FAQs)

How can I increase detection sensitivity with SuperSignal West Femto Maximum Sensitivity Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.
How can I increase detection sensitivity with SuperSignal West Dura Extended Duration Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.
What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other?

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Will the Pierce Western Blot Signal Enhancer interfere with protein detection?

The Enhancer does not bind to the membrane or proteins so it should not interfere with an antibody in binding the target protein.
Can I use the Pierce Western Blot Signal Enhancer on a stripped blot?

After stripping the blot, blocking reagent remains on the membrane so you will get inconsistent results. Therefore, we recommend using the Enhancer only before blocking and the first probing of the membrane.
Can I stain my membrane with Ponceau S prior to using the Pierce Western Blot Signal Enhancer?

We recommend using the Enhancer prior to staining and blocking.
Do I need to use a Pierce western blotting substrate with the Pierce Western Blot Signal Enhancer?

The Enhancer will work for any chemiluminescent or colorimetric substrate.

How does the Pierce Western Blot Signal Enhancer make the signal stronger?

The Enhancer relaxes the proteins, allowing the antibody to bind to the protein more efficiently.
When should I use the Pierce Western Blot Signal Enhancer?

The Pierce Western Blot Signal Enhancer should be used after transferring proteins to the membrane and before blocking or staining the membrane.

For Research Use Only. Not for use in diagnostic procedures.