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Fisherbrand™ Semidry Blotting Apparatus
Sleek, low-profile system for fast, efficient Western transfers
Supplier: Fisherbrand™ HEP1XMFIS
Includes: Lid with anode, base with cathode, connecting knobs, power cords
Description
Transfers minigels in 15 to 30 minutes and full-sized gels in 30 to 60 minutes, with efficiencies of nearly 90%.
- Multiple gels can be transferred by stacking gel/membrane/filter paper “sandwiches” Because plate electrodes are in direct contact with “sandwich,” a homogeneous electric field is created, ensuring even transfers System requires only minimal amounts of buffer (about 250mL) Low voltage—requires less than 1mA/cm2 of current; no external cooling is necessary
- Features 20 × 20cm platinum-coated titanium anode and stainless-steel cathode
- Transfers proteins from polyacrylamide electrophoresis gels onto hybridization membranes (Western transfers)
- Ideal for membrane-based, solid-phase protein assays such as HIV testing, where viral antigens are assayed with patient antibodies
- Use to detect trace proteins in complex mixtures and study protein interaction with other molecules (i.e., DNA)
Specifications
| 25 x 25 x 5 cm Polyacrylamide Electrophoresis Gel | |
| Lid with 20 x 20cm Platinum coated Titanium anode, base with Stainless Steel cathode, connecting knob, power cords | |
| 250 mL | |
| 9.75 in. | |
| 9.75 in. | |
| 2 in. | |
| 1 mA/cm2 |
| Semi Dry Electroblotting System | |
| 8.45 oz. | |
| 9.75 x 9.75 x 2 in. (24.76 x 24.76 x 5.08 cm) | |
| 24.76 cm | |
| 24.76 cm | |
| 5.08 cm |