
Real Time PCR Master Mixes
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Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Detection Method | SYBR |
Applied Biosystemsâ„¢ Power SYBRâ„¢ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBRâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal PCR Master Mix, no AmpEraseâ„¢ UNG
TaqManâ„¢ Universal PCR Master Mix, No AmpEraseâ„¢ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, GeneAmp 9600, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,miRNA analysis,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqManâ„¢ |
Invitrogenâ„¢ Platinumâ„¢ Quantitative PCR SuperMix-UDG
Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Product Type | Quantitative PCR SuperMix-UDG |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Platinumâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ EXPRESS qPCR Supermix, with premixed ROX
EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.
GC-Rich PCR Performance | High |
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Content And Storage | EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube. Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Format | Tube |
Product Type | Express qPCR Supermix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorfâ„¢ MasterCyclerâ„¢ RealPlex, StepOneâ„¢, Fast Mode, StepOneâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | Primer-probe |
Invitrogenâ„¢ EXPRESS qPCR Supermix, universal
EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.
GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | Express qPCR Supermix |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | Primer-probe |
Invitrogenâ„¢ Platinumâ„¢ Quantitative PCR SuperMix-UDG w/ROX
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.
Shipping Condition | Dry Ice |
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Polymerase | Platinum Taq DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | Quantitative PCR SuperMix-UDG |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Platinumâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqPathâ„¢ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System, 7500 Fast Dx System, QuantStudioâ„¢ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ PowerTrackâ„¢ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
Shipping Condition | Dry Ice |
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Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, Bio-Rad CFX Connectâ„¢, Bio-Rad CFX384 Touchâ„¢, Bio-Rad CFX96 Touchâ„¢, BioRad CFX96â„¢, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiAâ„¢ 7 System, StepOneâ„¢ System, MJ Chromo4, MJ Opticon, StepOnePlusâ„¢ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | Applied Biosystemsâ„¢ |
Applied Biosystemsâ„¢ TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix, no Rox
TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • Optimized buffer Store at 2–8°C. |
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Passive Reference Dye | None |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudioâ„¢ 12k Flex Dx, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, 7500 Fast Dx System, StepOneâ„¢ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Microbial Detection |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
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Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, 7500 Fast Dx System, StepOneâ„¢ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Microbial Detection |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ VetMAXâ„¢ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Polymerase | AmpliTaqâ„¢ Fast DNA Polymerase |
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Activation Time | 5 min. |
Final Product Type | Mastermix |
Form | Liquid |
Reaction Speed | Fast |
Label or Dye | ROX |
Hot Start | Built-in Hot Start |
Concentration | 2X |
Starting Material | DNA/RNA |
Compatibility | TaqManâ„¢ |
For Use With (Application) | Animal Health |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ Absolute Qâ„¢ Universal DNA Digital PCR Master Mix (5X)
Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |