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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (228)
Real Time PCR Reagents and Kits (183)

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1 – 15 of 407 results
1
Promotions Available

Applied Biosystems™ SYBR™ Select Master Mix for CFX  DFS Item

A cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Product Line SYBR
2
Promotions Available

Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)  DFS Item

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

GC-Rich PCR Performance High
Product Type PreAmp Master Mix
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type cDNA
For Use With (Application) Pre-amplification
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
3
Promotions Available

Applied Biosystems™ SYBR™ Select Master Mix  DFS Item

Cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
4

VetMAX™-Gold AIV Detection Kit  DFS Item

The VetMAX™-Gold AIV Detection Kit (Avian Influenza Virus RNA Test Kit) is a highly sensitive, qualitative, one-step, real-time RT-PCR assay to detect Avian Influenza Virus (AIV) RNA isolated from poultry (chicken/turkey) oropharyngeal/tracheal swab samples.

8
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG  DFS Item

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
9
Promotions Available

Applied Biosystems™ TaqMan™ Advanced miRNA Assay  DFS Item

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR.

10

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits
GREENER_CHOICE

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Human DNA
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
12
Promotions Available

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix  DFS Item

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple Very High
For Use With (Application) Genotyping
PCR Method qPCR
Multiplex Capability Up to 3-plex, Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath
13
Promotions Available

Applied Biosystems™ TaqPath™ ProAmp™ Multiplex Master Mix

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple Very High
For Use With (Application) Genotyping,Pharmacogenomics
PCR Method qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Product Type qPCR Master Mix
Passive Reference Dye Mustang Purple
For Use With (Equipment) QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7 Real-Time PCR System ViiA 7 Dx, 7500 Fast, StepOne or StepOnePlus
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath
14

Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.2 mL

A simple solution for dengue, chikungunya, and Zika virus RNA detection: just add RNA.

15

Applied Biosystems™ QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit  DFS Item

The QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit contains two training plates and a synthetic amplification control to serve as a sample for experiments.