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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (238)
Real Time PCR Assays and Arrays (229)
Real Time PCR Reagents and Kits (183)

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1

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type Master Mix
Form Frozen
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Detection Method SYBR
2

Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe
3
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Master Mix  DFS Item

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
4
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG  DFS Item

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
6
Promotions Available

Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - KRAS 517  DFS Item

Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.

7
Promotions Available

Applied Biosystems™ TaqMan™ Array Human Immune Response  DFS Item

TaqMan™ Array Human Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes.

8
Promotions Available

Applied Biosystems™ TaqMan™ Array Human Protein Kinase Pathways  DFS Item

TaqMan™ Array Human Protein Kinase Pathways 96-well Plate contains 92 assays to protein kinases and associated genes and 4 assays to candidate endogenous control genes.

9
Promotions Available

Applied Biosystems™ TaqMan™ Array Human Stem Cell Pluripotency  DFS Item

TaqMan™ Array Plates contain the same high quality TaqMan™ Gene Expression Assays that have been commercially available for years in a convenient, dried, 96-well format.

10
Promotions Available

Applied Biosystems™ TaqMan™ Array Human Hedgehog Pathway  DFS Item

TaqMan™ Array Human Hypoxia 96-well Plate contains 44 assays to hypoxia signaling associated genes and 4 assays to candidate endogenous control genes.

11
Promotions Available

Applied Biosystems™ TaqMan™ Array Human Hypoxia  DFS Item

TaqMan™ Array Human Hypoxia 96-well Plate contains 44 assays to hypoxia signaling associated genes and 4 assays to candidate endogenous control genes.