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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (239)
Real Time PCR Assays and Arrays (230)
Real Time PCR Reagents and Kits (182)

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1

Applied Biosystems™ TrueMark™ Urinary I Plus Combo Kit  DFS Item

The Applied Biosystems TrueMark Urinary I Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems.

2
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Applied Biosystems™ TaqMan™ Array Human Endogenous Control  DFS Item

TaqMan™ Express Endogenous Control Plates use TaqMan™ probe-based chemistry and are designed for use on the suite of Applied Biosystems™ Real-Time PCR Systems.

5
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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - EGFR 6225  DFS Item

Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.

7

Applied Biosystems™ TaqMan™ SCID/SMA Assay  DFS Item

A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control.

Shipping Condition Dry Ice
Content And Storage • DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Assay

Upon receipt, store at -20°C. After opening, store at 4°C.
For Use With (Equipment) QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Sample Type DNA (Genomic), cDNA
Product Line TaqMan
Detection Method Primer-probe
8
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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - EGFR 6213  DFS Item

Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.

11

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

12
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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG  DFS Item

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
13

Thermo Scientific™ Maxima Probe qPCR Master Mixes

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

14

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox  DFS Item

TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).

For Use With (Application) Microbial Detection
PCR Method qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Sensitivity Down to 1 copy
Passive Reference Dye None
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 12k Flex Dx, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, 7500 Fast Dx System, StepOne System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Very High
Target Specificity cDNA or gDNA only
Product Line TaqPath
15
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Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at -15°C to -25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 5-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath