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Reverse Transcription Products

A variety of kits, reagents, and mixes suitable for reverse transcription processes. Products include one-step RT-PCR reagent, cDNA synthesis kits and master mixes, and standalone reverse transcriptases.
cDNA Synthesis Kits and Master Mixes (63)
Reverse Transcriptases (24)
One-Step Reverse Transcription PCR Reagents (13)

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1 – 15 of 42 results
1

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 7 kb
Reaction Format Premixed Components
Final Product Type First-Strand cDNA
Reaction Speed 30 to 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 37°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
2

Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 15 to 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
3

Thermo Scientific™ Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

Shipping Condition Wet or Dry Ice
Format Kit
Reverse Transcriptase Verso
Size (Final Product) Up to 11 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
4

Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.

Concentration 200 U/μL
5

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
6

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Shipping Condition Dry Ice
Content And Storage

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
7

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
8

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase RevertAid H Minus
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
9

Invitrogen™ MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

10

Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents

The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.

11

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Format Super Mix
Reverse Transcriptase SuperScript III
Reaction Format Master Mix
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
12

Invitrogen™ SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
13

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
14

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR

The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

15

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Shipping Condition Wet Ice
Format Kit
Reverse Transcriptase SuperScript™ II
Final Product Type cDNA (Double-Stranded)
Product Type cDNA Synthesis Kit
Sample Type RNA
Labeling Method Direct Labeling
Optimal Reaction Temperature 42°C
For Use With (Application) Microarray Analysis
Product Line SuperScript