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Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.
Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.
Type | Transfection Reagent |
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Part of the luciferin/luciferase light-producing reaction
Drive Cypridina luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
Drive red-shifted firefly luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters
Compatible Cells | Mammalian Cells |
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Substrate Type | Luciferase Substrate |
Detection Method | Bioluminescence |
Promoter | EF-1α |
Substrate | Coelenterazine |
Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Gaussia Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Product Line | Pierceâ„¢ |
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.
Substrate | D-Luciferin |
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Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Firefly Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Compatible Cells | Mammalian Cells |
Substrate Type | Luciferase Substrate |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Product Line | Pierceâ„¢ |
Detection Method | Bioluminescence |
Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.
Substrate | Coelenterazine, D-Luciferin |
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Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Firefly Luciferase, Gaussia Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Compatible Cells | Mammalian Cells |
Substrate Type | Luciferase Substrate |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Product Line | Pierceâ„¢ |
Detection Method | Bioluminescence |
Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.
Perform firefly luciferase assays in high-throughput screening applications.
Substrate | D-Luciferin |
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Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Firefly Luciferase, Cypridina Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Compatible Cells | Mammalian Cells |
Substrate Type | Luciferase Substrate |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Detection Method | Bioluminescence |
Drive Cypridina luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
Substrate | Vargulin (Cypridina Luciferin), D-Luciferin |
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Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Cypridina Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Compatible Cells | Mammalian Cells |
Substrate Type | Luciferase Substrate |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Product Line | Pierceâ„¢ |
Detection Method | Bioluminescence |