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Thermo Scientific™ pCMV-Cypridina Luc Vector for Luciferase Assays

Numéro de catalogue. PI16150
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10 μg
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Numéro de catalogue. Quantity
PI16150 10 μg
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Numéro de catalogue. PI16150 Fournisseur Thermo Scientific™ Code fournisseur LSG16150
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Description

Drive Cypridina luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

The Thermo Scientific™ pMCS-Cypridina Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.

The Cypridina Luc Vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca. The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Naturally-secreting Cypridina luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Spécifications

Description pCMV-Cypridina Luc Vector
Type Luc Vector
Cloning Method Restriction Enzyme/MCS
Delivery Type Transfection
Quantity 10 μg
Content And Storage Store between -70°C and -20°C.
Reporter Gene Cypridina Luciferase
Selection Agent (Eukaryotic) Puromycin
Product Line Pierce
For Use With (Application) Reporter Assays
Promoter CMV
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For Research Use Only. Not for use in diagnostic procedures.