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Thermo Scientific™ pTK-Gaussia Luc Vector for Luciferase Assays

Catalog No. PI16148
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PI16148 10 μg
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Catalog No. PI16148 Supplier Thermo Scientific™ Supplier No. 16148
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Description

Drive Gaussia luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

The Thermo Scientific™ pMCS-Gaussia Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Specifications

Description pTK-Gaussia Luc Vector
Type Luc Vector
Cloning Method Restriction Enzyme/MCS
Delivery Type Transfection
Quantity 10 μg
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Reporter Gene Gaussia Luciferase
Selection Agent (Eukaryotic) Puromycin
Product Line Pierce
For Use With (Application) Reporter Assays
Promoter TK
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For Research Use Only. Not for use in diagnostic procedures.