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Protein Labeling Reagents

Various reagents designed to selectively label target proteins. Products include classic dyes, radioisotope labels, biotinylation reagents, fluorescent labels, molecular probes, assays, and labeling kits.
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1

Thermo Scientific™ DyLight™ 488 NHS Ester

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.

Labeling Target Proteins
Content And Storage Store desiccated at -20°C, protected from light.
Product Type Dye
Label or Dye DyLight™ 488
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide)
Emission 518
Excitation 493
Label Type DyLight Fluors
Chemical Reactivity Amine
Product Line DyLight
2

Thermo Scientific™ EZ-Link™ Biotin-LC-Hydrazide

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (24.7A) hydrazide-activated biotinylation reagent.

3

Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Content And Storage Store desiccated at -20°C, protect from moisture
Product Type NHS-Fluorescein
Label or Dye FITC (Fluorescein)
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide)
Emission 518
Excitation 494
Label Type Classic Dyes
Chemical Reactivity Amine
Product Line Pierce
4

Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)  DFS Item

New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay.

5

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Chemical Reactivity Amine
6

Thermo Scientific™ EZ-Link™ NHS-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.

7

Thermo Scientific™ Pierce™ Dye Removal Columns

Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.

9

Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin, No-Weigh™ Format

Biotinylate reduced cysteines with this short, maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a 2-unit PEG spacer arm to enhance water solubility.

Cell Permeability Cell-Impermeant
Product Type Maleimide-PEG2-Biotin
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Reactive Moiety Maleimide
Chemical Reactivity Thiol
Product Line EZ-Link
10

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Cell Permeability Cell-Impermeant
Product Type Sulfo-NHS-LC-Biotin
Spacer Mid-length
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
11

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

Cell Permeability Cell-Impermeant
Content And Storage Store at -20°C.
Product Type NHS-PEG4-Biotin
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
13

Thermo Scientific™ EZ-Link™ NHS-Biotin

Biotinylate protein amines (e.g., lysines) with a simple, short (13.5A), NHS-ester biotinylation reagent containing a membrane-permeable spacer arm for intracellular labeling.

14

Invitrogen™ Alexa Fluor™ 430 NHS Ester (Succinimidyl Ester)  DFS Item

Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 430 dye is water soluble and pH-insensitive from pH 4 to pH 10.

15

Molecular Probes™ Alexa Fluor™ 594 Azide (Alexa Fluor™ 594 Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), mixed isomers  DFS Item

Alexa Fluor™ 594 Azide (Alexa Fluor™ 594 Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), mixed isomers