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Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Numéro de catalogue. PI26136
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100 mg
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Numéro de catalogue. Quantity
PI26136 100 mg
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Numéro de catalogue. PI26136 Fournisseur Thermo Scientific™ Code fournisseur 26136
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Description

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this long (53.2A) biotinylation reagent containing an 11-unit polyethylene glycol spacer.

Thermo Scientific™ EZ-Link Amine-PEG11-Biotin is a biotinylation reagent containing a long 11-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine group for conjugation and protein labeling with EDC or other crosslinkers.

EZ-Link Amine-PEGn-Biotin reagents are water-soluble, polyethylene glycol (PEG)-containing reagents with terminal primary amines (NH2). The PEG spacer arm is hydrophilic and confers greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The amine group of these reagents can be reacted with carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the NH2 group of the amine-biotin, forming an amide bond.

Highlights:

  • Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin

  • Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers

  • Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies

  • Spacer arm – the reach between the point of attachment and the biotin affinity tag is more than 50 angstroms, minimizing steric hindrance for the biotin binding-function

Specifications:

  • Chemical name (simplified): biotinyl-undecaoxapentatriacontanediamine
  • Chemical formula: C34H66N4O13S
  • Molecular weight: 770.97
  • Spacer arm: 53.2 angstroms
  • Form: white to off-white solid
  • Solubility: at least 25mg/mL in water or buffer

Spécifications

Product Type Amine-PEG11-Biotin
Quantity 100 mg
Content And Storage Store at -20°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Carboxylic Acid, Ketone, Aldehyde
Reactive Moiety Amine
Solubility Water
Label or Dye Biotin
Spacer Extra-long, Pegylated
Label Type Biotin & Analogs
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For Research Use Only. Not for use in diagnostic procedures.