Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications.
MultiShot StripWell Stbl3 Chemically Competent E. coli cells are cloning competent cells designed for cloning direct repeats found in lentiviral expression vectors.