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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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Promotions Available

Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells  DFS Item

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.

2

Invitrogen™ One Shot™ BL21(DE3)pLysE Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysE Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

3

Invitrogen™ TOP10 Electrocomp™ Kit  DFS Item

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

4

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

5

MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: 2 x 106 cfu/μg
6

Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli  DFS Item

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

7

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cell Set

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

8

Gibco™ FreeStyle™ CHO Expression Medium  DFS Item
GREENER_CHOICE

Animal origin-free, chemically defined, protein-free medium

Cell Line FreeStyle™ CHO-S Cells, CHO
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Product Type Expression Medium
Endotoxin Level ≤0.25 EU/mL, ≤ 0.25 EU/mL
Classification Chemically Defined, Protein-Free, Serum-Free
Culture Type Suspension Cell Culture
Phenol Red Indicator No Phenol Red
Product Line Freestyle, Gibco
Serum Level Serum-Free
Glutamine No Glutamine
9

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli  DFS Item

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
10

Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

Shipping Condition Dry Ice
Blue/White Screening No
Cloning Methylated DNA No
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
ToxicProteins No
High-throughput Compatibility Low
Improves Plasmid Quality No
ImprovesProteinStability Yes (lon, ompT)
Promoter T7
Preparing Unmethylated DNA Yes (dcm)
Reduces Recombination No
ImprovesRNAStability Yes (pLysS)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Chloramphenicol)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (B)
Product Line One Shot
Contains F' Episome No
11

Invitrogen™ One Shot™ BL21(DE3) Chemically Competent E. coli  DFS Item

One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

12

Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli  DFS Item

One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.

13

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli  DFS Item

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

14

Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli  DFS Item

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
High-throughput Compatibility High
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Product Type Chemically Competent Cells
Product Line MultiShot
Contains F' Episome No
15

Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells  DFS Item

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.