Modifying Enzymes
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Invitrogen™ TrueCut™ Cas9 Protein v2
A next-generation CRISPR Cas9 protein engineered to deliver maximum on-target editing efficiency. TrueCut Cas9 Protein v2 is ideal for applications requiring the highest editing efficiency.
Invitrogen™ Anza™ Alkaline Phosphatase
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
MilliporeSigma™ RNase A
As supplied with Montage™ Plasmid MiniprepHTS 96 Kit
Invitrogen™ T4 DNA Ligase (5 U/μL)
Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini
Thermo Scientific™ E. coli DNA Ligase
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Thermo Scientific™ Endonuclease V, T.maritima (5 U/μL)
Degrade DNA with Endonuclease V, T. maritima (Endo V), a 3'-endonuclease involved in DNA repair that initiates removal of deaminated bases from damaged DNA.
Invitrogen™ Anza™ T4 DNA Ligase Master Mix
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Invitrogen™ E. coli DNA Ligase
Catalyzes formation of phosphodiester bonds in presence of β-NAD between double-stranded DNAs with 3' hydroxyl and 5' phosphate cohesive termini