Modifying Enzymes

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Fisher Scientific Edge
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Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

Thermo Scientific™ Proteinase K, recombinant, PCR grade

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade  

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

MP Biomedicals™ Hyaluronidase, Bovine Testes

Bovine Testes

Gibco™ Human MRP4 Vesicles  

Human MRP4 Vesicles

Thermo Scientific™ T4 beta-glucosyltransferase

Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis

Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Thermo Scientific™ Micrococcal Nuclease (300 U/μL)

Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.

MilliporeSigma™ Calbiochem™ Y-27632

Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

Thermo Scientific Chemicals Deoxyribonuclease II, porcine spleen

Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile

Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone.

Invitrogen™ Topoisomerase I  

Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds

Thermo Scientific™ RNase T1 (1000 U/μL)

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Invitrogen™ Proteinase K Powder  

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.