Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.

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Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Pricing and Availability
Specifications

Content And Storage	-20°C
Form	Solid
Product Type	Proteinase K
Color	White
For Use With (Application)	Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source	Tritirachium album

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site	A.AGCTT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	HindIII
Storage Buffer	10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Pricing and Availability
Specifications

Components	>50% Water, 25 to 50% Glycerin
Cut Site	AT.CGAT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	ClaI (Bsu15I)
Storage Buffer	10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL

Components	25 to 50% Water, >50% Glycerin
Cut Site	GC.GGCCGC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	NotI
Storage Buffer	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Concentration	10 U/μL
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

OPTIZYME™ HpaII, Fisher BioReagents™

Pricing and Availability

OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

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OPTIZYME™ BglII, Fisher BioReagents™

Pricing and Availability
Specifications

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site	A.GATCT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	BglII
Storage Buffer	10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII

OPTIZYME™ SacI, Fisher BioReagents™

Pricing and Availability

OPTIZYME™ SmaI, Fisher BioReagents™

Pricing and Availability
Specifications

Components	10X OPTIZYME™ Buffer 4
Cut Site	CCC.GGG
Form	Liquid
Product Type	SmaI
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	30°C
Concentration	10 U/μL
For Use With (Application)	>90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI

Invitrogen™ DQ™ Red BSA - Special Packaging

Hydrolysis of substrate yields brightly fluorescent product

Pricing and Availability

Thermo Scientific™ AluI (10 U/μL)

Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Alu I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

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