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Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (593)
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Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

EDGE
Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
2

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade  DFS Item

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

3

OPTIZYME™ NotI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site GC.GGCCGC
Content And Storage Keep container tightly closed
Form Liquid
Product Type NotI
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Concentration 10 U/μL
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
4

OPTIZYME™ BglII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site A.GATCT
Content And Storage Keep container tightly closed
Form Liquid
Product Type BglII
Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
5

Thermo Scientific™ FastDigest PstI

The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).

Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Pst I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 3' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest
6

Thermo Scientific™ FastDigest XhoI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Xho I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 80°C
Product Line FastDigest
7

Invitrogen™ T4 DNA Ligase (1 U/μL)  DFS Item

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Product Type T4 DNA Ligase
Enzyme Ligase
Concentration 1 U/μL
8

Thermo Scientific™ FastDigest SfiI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

9

Thermo Scientific Chemicals Peroxidase, horseradish

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

Form Lyophilized
10

Invitrogen™ TURBO DNA-free™ Kit  DFS Item

Ideal tool for clearing residual DNA

11

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Product Type Alkaline Phosphatase
Enzyme Phosphatase
Compatible Buffer PCR Buffer, Enzyme Buffer
Concentration 1 U/μL
12

Thermo Scientific™ FastDigest Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).

Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Eco32I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang Blunt
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest
14

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

15

Thermo Scientific™ RNase T1 (1000 U/μL)

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Product Type RNase T1
Enzyme RNase
Concentration 1000 U/μL