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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Type de cellule Chemically Competent
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
2

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
3

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Each kit uses the pCR™4Blunt-TOPO; vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, T3
Méthode de clonage Blunt TOPO™
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
4

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Souche bactérienne ou de levures TOP10F ́
Format Kit
Type de produit Cloning Kit
Vecteur pCRII
Gamme de produits One Shot
Comprend Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Type de cellule Chemically Competent E. coli
Accélérateur T7, SP6
Méthode de clonage TA Cloning
À utiliser avec (application) PCR Cloning
Contenu et stockage This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
5

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli  DFS Item

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

6

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli  DFS Item

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

7

Invitrogen™ Zero Blunt™ PCR Cloning Kit  DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits Zero Blunt
Accélérateur T7
Méthode de clonage Blunt PCR
Contenu et stockage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
8

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells  DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Souche bactérienne ou de levures Not Included
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits TOPO, Zero Blunt
Accélérateur T7
Méthode de clonage Blunt PCR
Contenu et stockage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
9

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Type de cellule Chemically Competent
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
10

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

11

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning  DFS Item

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

12

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

14

Invitrogen™ TOPO™ TA Kit, TOP10F' (Supply Center Packaging)  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

15

Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Souche bactérienne ou de levures DH5α
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.