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Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit 
Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.
Supplier: Gibco™ A14697
Description
- Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
- Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
- WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
- TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
- Neomycin resistance gene for selection of stable cell lines with Geneticin™
- pUC origin for high copy replication and maintenance of the plasmid in E. coli
Specifications
| Ampicillin (AmpR) | |
| Constitutive | |
| Transfection | |
| High Copy Plasmid | |
| TA Cloning Kit | |
| Untagged | |
| pUC Origin | |
| NeoR | |
| pcDNA |
| TOPO™-TA | |
| Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C. Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C. |
|
| Protein Production, Constitutive Expression | |
| TOPO™ | |
| CMV | |
| 20 μg Kit | |
| Geneticin™ (G-418) | |
| SV40 Promoter |
For Research Use Only. Not for use in diagnostic procedures.