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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells  DFS Item

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

2

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

3

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

4

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

5

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

6

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

7

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 1 (untagged)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

8

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

9

Thermo Scientific™ aLICator LIC Cloning and Expression Set 2 (All-in-One/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

10

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix  DFS Item

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Shipping Condition Ambient, Wet Ice, Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
11

Invitrogen™ TrueTag™ Donor DNA Kit, BFP  DFS Item

The Invitrogen TrueTag Donor DNA Kit, BFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

12

Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Product Type Rapid DNA Ligation Kit
Enzyme DNA Ligase
13

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

14

Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit  DFS Item

The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.