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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (220)
DNA Synthesis Reagents and Columns (107)
PCR Cloning Kits (104)
DNA Cloning and Mapping and Mutagenesis (83)
Plasmid DNA (6)

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2

Invitrogen™ RNase T1 (1 U/μL)  DFS Item

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

3

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Résistance aux antibiotiques des bactéries Ampicillin (AmpR)
Format Kit
Vecteur pJET1.2/blunt
Gamme de produits CloneJET
Type d’échantillon PCR Products
Méthode de clonage Restriction Enzyme⁄ MCS, Blunt PCR
À utiliser avec (application) PCR Cloning
4

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

5

Thermo Scientific™ pBR322 Plasmid DNA

Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.

6

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

7

Thermo Scientific™ Jurkat Genomic DNA

Use this pure human genomic DNA as a control in DNA methylation analysis, genomic DNA library construction, PCR, and Southern blotting.

8

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

9

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

10

Applied Biosystems™ Control DNA (from CEPH Individual 1347-02)  DFS Item

The control DNA can be used with linkage mapping sets MD10, HD5, and LD20.

12

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells  DFS Item

Résistance aux antibiotiques des bactéries Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Type de produit PCR Cloning Kit
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
13
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

14

Applied Biosystems™ 3730 Running Buffer (10X)  DFS Item
GREENER_CHOICE

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.