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Applied Biosystems™ 3730 Running Buffer (10X)
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Catalog No. 4335613
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Catalog No. 4335613 Supplier Applied Biosystems™ Supplier No. 4335613
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Description

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™ 3730 and 3730xl DNA Analyzers.

Note: For Research Use Only. Not for use in diagnostics procedures.

Specifications

Buffer Running Buffer
Concentration 10 X
Content And Storage Store at 2°C to 8°C.
For Use With (Equipment) 3730xl DNA Analyzer, 3730 DNA Analyzer
For Use With (Application) Standard Sequencing
Product Type 3730 Running Buffer
Quantity 500 mL
Form Liquid

Frequently Asked Questions (FAQs)

What is the appropriate volume of liquid for use in the buffer, water, and waste reservoirs, on the Applied Biosystems 3730 Series DNA Analyzer?

The appropriate volume to use in the buffer, water, and waste reservoirs is 80mls. The black line on the reservoir body is a reference only and should not be used to determine the fill volume. For accuracy and reproducibility in filling the reservoirs, a graduated cylinder should be used each time the reservoir fluids are replaced.
How long can the 1x Running Buffer for the 3730 Series be stored?

The 1x Running Buffer can be stored for 1 week at room temperatures (20–25°C) or for 1 month at refrigerated temperatures (2–8°C).
How long can Running Buffer stay on the Applied Biosystems 3730 Series DNA Analyzer?

The Running Buffer on the Applied Biosystems 3730 Series DNA Analyzer has a use-life of 48 hours on the instrument. Replace the Running Buffer (in both the buffer jar and reservoirs) every 48 hours.

When I started my run on the Applied Biosystems 3730/3730xl DNA Analyzer, an error occurred: "Leak detected" but I don't see any leaks. What else might cause this?

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to either an improperly tightened or improperly threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons are:

1.Valve stuck in open position
2.Large air bubble under the piston
3. Polymer:
-Wrong polymer type (e.g., instrument thinks it has POP-7 on it but it really has POP-4).
-Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.
When I started my run on the Applied Biosystems 3730/3730xl DNA Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2.Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3.Using Running Buffer that was:
-Diluted to 1X incorrectly
-Swapped with the water position on the
Autosampler deck
-On the instrument longer than 48 hours
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.
I have an Applied Biosystems 3730/3730xl DNA Analyzer. When do my consumables expire?

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date of shipment.

Can I use Applied Biosystems 3730 DNA Analyzer 10X Running Buffer on the Applied Biosystems 3130 Genetic Analyzer?

We do not recommend mixing and matching the various instrument reagents as each is manufactured using different processes, tested as an entire system, and warrantied to perform to a minimum specification. Substitution of reagents can compromise the integrity of the system and cannot be supported by our service or support specialists. In addition, the 10X Running Buffer expires 1 year after date of shipment and once diluted to 1X, it will last 30 days at 2-8 degree C or 7 days at ambient temperature. This could potentially result in having to dispose of a large volume of 10X Running Buffer due to expiration if you are using it on a lower-throughput system.

My consumables for the Applied Biosystems 3730/3730xl DNA Analyzer have expired, but how long past expiration can I really use them?

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.
Do I need to replace the buffer every 2-3 days if the Applied Biosystems 3730/3730xl DNA Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 2-3 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer with freshly made 1X Running Buffer prior to starting a new run.
How long can I keep the buffer on the Applied Biosystems 3730/3730xl DNA Analyzer?

For the Applied Biosystems 3730/3730xl DNA Analyzer, the buffer can keep for 48 hours.

For Research Use Only. Not for use in diagnostic procedures.