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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

MP Biomedicals™ Peptone T (Tryptic Digest of Tissue)

Intended for use in fermentation, animal feeds and other uses in which the insoluble particles will be of no consequence.

2

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent  DFS Item

StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware.

3

Gibco™ Trypsin-EDTA (0.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

4

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Shipping Condition Wet Ice
Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality 280 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
6

Gibco™ Trypsin (2.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

7

Gibco™ Trypsin-EDTA (0.05%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
8

Gibco™ Trypsin-EDTA (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
9

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red  DFS Item
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
10

Gibco™ TrypLE™ Select Enzyme (1X), no phenol red  DFS Item

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Phenol Red
Shelf Life 24 Months
Concentration 1 X
11

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
12

Gibco™ TrypLE™ Express Enzyme (1X), phenol red  DFS Item
GREENER_CHOICE

Animal origin-free, recombinant enzyme

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
13

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

14

Gibco™ Trypsin Neutralizer Solution  DFS Item
GREENER_CHOICE

Sterile, phosphate-buffered saline solution

15

Gibco™ Trypsin/EDTA Solution (TE)  DFS Item
GREENER_CHOICE

With EDTA and phenol red