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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

2

Gibco™ Collagenase, Type II, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

3

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Shipping Condition Wet Ice
Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality 280 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
4

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
5

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Content And Storage 4°C
Formulation Chelating and stabilizing agents
6

Gibco™ Trypsin-EDTA (0.05%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
7

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
8

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

9

MilliporeSigma™ Chemicon™ Accumax™ Cell Detachment Solution

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

10

Gibco™ Trypsin-EDTA (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
11

Gibco™ Trypsin Neutralizer Solution  DFS Item
GREENER_CHOICE

Sterile, phosphate-buffered saline solution

12

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Content And Storage -20°C
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Calcium,No Magnesium,No Sodium Bicarbonate
pH 7 to 7.6
Sterility Sterile
13

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

14

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

15

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.