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Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)

Catalog No. 4379876
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Catalog No. 4379876 Supplier Applied Biosystems™ Supplier No. 4379876
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Description

Includes

Contains 2 x 75mL of GeneAmp™ 10X PCR Buffer I (500mM KCl, 100mM Tris-HCl, pH 8.3; 15mM MgCl2 and 0.01% (w/v) gelatin). Autoclaved.

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification. GeneAmp 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq DNA Polymerase.

GeneAmp 10X PCR Buffer I is available in several convenient sizes and when used with AmpliTaq DNA Polymerase offers maximum PCR performance.

Note

See user's manual or package insert for limited label license and trademark information.

Order Info

Shipping Condition: Wet ice

Specifications

Concentration 10X
Content And Storage • GeneAmp 10X PCR Buffer I (with 15 mM MgCl2), 2 x 75 mL

Store at -15°C to -25°C.
Polymerase AmpliTaq DNA Polymerase
Product Type PCR Buffer
Quantity 2 x 75 mL
Shipping Condition Wet Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Reaction Format Kit
Volume 150 mL

Frequently Asked Questions (FAQs)

How many freeze cycles do you recommend for Applied Biosystems, GeneAmp 10X PCR Buffer I (Cat. No. 4379876)?

We do not have specific guidance on the number of freeze thaws. We recommend following the guidance for aliquoting 1.5 mL of this buffer into autoclaved 2 mL tubes after the first thaw as described in Product Insert: GeneAmp 10X PCR Buffer I. This will help increase efficiency when working with the product and serve to limit the number of freeze-thaws for the buffer.

What are the differences between GeneAmp 10X PCR Buffer I, PCR Buffer II and PCR Gold Buffer, and what are the formulations of each buffer?

The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been carefully optimized for use with the AmpliTaq Gold enzyme. GeneAmp 10X PCR Buffer I contains Mg; Geneamp 10X PCR Buffer II and Geneamp 10X PCR Gold Buffer do not have Mg in them, but come with a separate vial of 25 mM MgCl2 so that you can titrate the Mg in your PCR reaction.

Buffer formulations:
Buffer I (500 mM KCl, 100 mM Tris-HCl, pH 8.3, 15 mM MgCl2, 0.01% (w/v) gelatin)
Buffer II (500 mM KCl, 100 mM Tris-HCl, pH 8.3), with separate tube of 25 mM MgCl2
Gold Buffer (500 mM KCl, 150 mM Tris-HCl pH 8.0), with separate tube of 25 mM MgCl2

For Research Use Only. Not for use in diagnostic procedures.