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Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)

Catalog No. 4403081
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Catalog No. 4403081 Supplier Applied Biosystems™ Supplier No. 4403081

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Reagents sufficient for 4,000 sample preparation reactions at 50μL/ reaction and 4,000 PCR reactions at 5μL for each reaction.

Go from Biological Sample to Genotyping Results in less than one hour.

The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

  • DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
  • The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
  • Compatible with both Fast and Standard mode thermal cycling conditions
  • Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
  • Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
  • Extended PCR cycling for poorly amplified or sample-limited reactions

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage Contains Lysis Reagents (2° to 8°C): 200mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C): 10mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
For Use With (Equipment) 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp™ 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti™ Thermal Cycler
Passive Reference Dye ROX (Pre-mixed)
Quantity 1 Kit
Target SNPs (Known), SNPs (Unknown or Numerous)
For Use With (Application) Genotyping
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood

For Research Use Only. Not for use in diagnostic procedures.