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Applied Biosystems™ TaqMan™ DNA Template Reagents

Catalog No. 401970
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Catalog No. 401970 Supplier Applied Biosystems™ Supplier No. 401970
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Includes

Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

TaqMan™ beta-actin Template Reagents can be used with TaqMan™ PCR Core Reagents or SYBR™ Green PCR Core Reagents for polymerase chain reaction (PCR).

  • Pre-designed primers and TaqMan™ probe eliminate assay design
  • Rapid assay development guidelines can minimize optimization time
  • Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included: We have developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.
Normalization Built-In: All our Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Order Info

Shipping Conditions: Dry Ice

Recommended Storage

Store at -20°C.

Specifications

Content And Storage Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer. Store at -20°C
Description Internal Probe Modification: FAM (5'), TAMRA Quencher (3')
Detection Method Primer-probe
Format Tube
Label or Dye FAM
Quantity 1 Kit
Target β-actin
For Use With (Application) Real Time PCR (qPCR)

For Research Use Only. Not for use in diagnostics procedures.