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Applied Biosystems™ TaqMan™ DNA Template Reagents DFS Item

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

Manufacturer:  Applied Biosystems™ 401970

Catalog No. 401970



Includes: Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer

Description

Description

TaqMan™ beta-actin Template Reagents can be used with TaqMan™ PCR Core Reagents or SYBR™ Green PCR Core Reagents for polymerase chain reaction (PCR).

  • Pre-designed primers and TaqMan™ probe eliminate assay design
  • Rapid assay development guidelines can minimize optimization time
  • Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included: We have developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.
Normalization Built-In: All our Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Order Info

Shipping Conditions: Dry Ice

Recommended Storage

Store at -20°C.

Specifications

Specifications

Internal Probe Modification: FAM (5'), TAMRA Quencher (3')
Tubes
Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer
Store at -20°C
Real time PCR (qPCR)
Primer-Probe Detection
FAM™
1 Kit
β-actin
SDS
Documents
Product Certifications
Promotions

Promotions

For Research Use Only. Not for use in diagnostics procedures.