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Standard PCR Reagents and Kits

Products for use during standard PCR procedures. Includes master mixes, DNA polymerases, reverse transcription mixes, PCR beads, buffers, and kits. Ideal when specificity; precise quantification; and high fidelity, sensitivity, and yield are not requirements.
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1 – 15 of 45 results
1

Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)  DFS Item

Enzyme removes any uracil incorporated into single- or double-stranded DNA

2

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Content And Storage Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
3

Applied Biosystems™ Fast MicroSEQ™ 500 16S rDNA PCR Kit  DFS Item

The Fast MicroSEQ™ 500 16S rDNA PCR kit utilizes new Fast technology and provides bacterial identifications from colony to results in as little as 5 hours using the Veriti™ 96-well Thermal Cycler.

4

Invitrogen™ Taq DNA Polymerase, native  DFS Item

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Standard PCR
Exonuclease Activity 5' - 3'
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
5

Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl2  DFS Item

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.

Polymerase AmpliTaq Gold DNA Polymerase
Format Kit
Product Type 10X PCR Gold Buffer
Concentration 10X
For Use With (Application) Standard PCR
6

Applied Biosystems™ AmpliTaq Gold™ 360 Buffer Kit  DFS Item
GREENER_CHOICE

The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.

7

Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with or without protocol  DFS Item

The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.

GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq DNA Polymerase
No. of Reactions 250 Reactions
Format Tube
Volume 0.1 mL or 0.2 mL
Size (Final Product) 2 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
8

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

9

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Polymerase AmpliTaq DNA Polymerase
Fidelity (vs. Taq) 1X
Reaction Format Kit
Product Type PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
10
Promotions Available

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
11

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Fidelity (vs. Taq) 1X
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Overhang 3'-A
Concentration 5 U/μL
Starting Material DNA
Exonuclease Activity 5' - 3'
12
Promotions Available

Applied Biosystems™ TaqMan™ DNA Template Reagents  DFS Item

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

13
Promotions Available

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe
14

Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.

15

Applied Biosystems™ MicroSEQ™ Full Gene 16S rDNA PCR Kit
GREENER_CHOICE

The MicroSEQ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria.