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Invitrogen™ Taq DNA Polymerase, native

Catalog No. p-4919380
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100 Units
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Catalog No. Quantity
18038018 100 Units
18038042 500 Units
18038067 3 x 500 Units
18038240 5000 Units
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Catalog No. 18038018 Supplier Invitrogen™ Supplier No. 18038018
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Description

Includes

Contains: 20μL Taq DNA Polymerase (5U/μL); 1.25mL 10X PCR buffer (without magnesium); 1mL Magnesium Chloride (50mM)

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

Concentration 5 U/μL
Content And Storage • Taq DNA Polymerase (5 U/μL), 20 μL
• 10X PCR buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL

Store at -20°C in a non-frost-free freezer.
Stable for 6 months when stored properly.
Format Stand-alone enzyme
GC-Rich PCR Performance Low
PCR Method qPCR, Standard PCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 100 Units
Shipping Condition Wet or Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 100 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.