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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Purity or Quality Grade LD (Low DNA)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Standard
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT, SYBR
2

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

3

Applied Biosystems™ SNaPshot™ Multiplex Kit  DFS Item

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Product Type Multiplex Kit
For Use With (Equipment) 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type DNA
Technique SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line SNaPshot
Detection Method Primer-probe
4

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

5

Applied Biosystems™ MicroSEQ™ 500 16S rDNA PCR Kit
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The MicroSEQ™ 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ™ 500 16S rDNA Sequencing Kit.

6

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.

Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
7

Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

8

Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit  DFS Item

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

9

Applied Biosystems™ QuantStudio™ 12K Flex OpenArray™ Calibration Kit  DFS Item

Offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray™ block type.

10

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)  DFS Item

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

11

Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

12

Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

13

Thermo Scientific™ Template Generation System II Kit

Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.

14

Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer  DFS Item

These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.