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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit  DFS Item
GREENER_CHOICE

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

Compatibilité à haut débit Not High-throughput Compatible (Manual)
Format Kit
Technologie d’isolement ChargeSwitch™ Chemistry
Cible DNA from Enzymatic Reactions (e.g. PCR)
Type de produit PCR Clean-Up Kit
Gamme de produits ChargeSwitch
Type d’échantillon PCR Products
Conditions d’expédition Room Temperature
À utiliser avec (application) Next-Generation Sequencing (NGS),Cloning,Standard PCR
Contenu et stockage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.
2

Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

3

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads  DFS Item

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

4

Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)  DFS Item

The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.

5

Invitrogen™ Single Cell Lysis Kit  DFS Item
GREENER_CHOICE

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

6

Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit  DFS Item

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

7

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Compatibilité à haut débit Yes
Méthode de détection TaqMan
Format Kit
Technologie d’isolement Direct cell lysis
Cible cDNA
Type d’échantillon Mammalian Cells
Cible de purification RNA
Méthode PCR 2-step RT-qPCR
Matériau de départ 10-100,000 Cells
Conditions d’expédition Frozen, Dry Ice
Type de kit Direct Cell Lysis RT-qPCR
À utiliser avec (application) Gene Expression
Contenu et stockage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
8

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Méthode de détection Primer-probe
À utiliser avec (équipement) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Vitesse de réaction Standard
Type de produit Gene Expression Cells-to-C Kit
Colorant de référence passive ROX (Pre-mixed)
Pureté ou qualité UP (Ultra Pure)
Type d’échantillon Cell
Méthode PCR 2-step RT-qPCR
Conditions d’expédition Dry Ice
Polymérase DNA Polymerase
Format Tube
Gamme de produits Ambion, Cells-to-CT, TaqMan
Transcriptase inverse M-MLV
10

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

GC-Rich PCR Performance Medium
Vitesse de réaction Fast
Format de réaction SuperMix or Master Mix
Type de produit Direct PCR Master Mix
Type d’échantillon Tissue
En surplomb Blunt
Méthode PCR Direct PCR
Concentration 2X
Conditions d’expédition Wet or Dry Ice
À utiliser avec (application) Genotyping,Hot-start PCR
Comprend l’étiquette ou le colorant Yes
Polymérase Phire Hot Start II DNA Polymerase
Hot Start Built-In Hot Start
Matériau de départ DNA
11

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

12

Invitrogen™ Precision gRNA Synthesis Kit  DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

13

Invitrogen™ PureLink™ PCR Purification Kit  DFS Item

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Durée de fonctionnement 10 min.
Format Kit
Technologie d’isolement Silica Spin Column
Type d’échantillon PCR Products
Conditions d’expédition Room Temperature
Contenu et stockage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
14

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

15

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.