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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (649)
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1 – 15 of 724 results
1
Promotions Available

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
4

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
5

Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)  DFS Item

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

6

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase  DFS Item

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq DNA Polymerase
Format One-Step RT-PCR System
Size (Final Product) Up to 4.5 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
7

Invitrogen™ dTTP (100 mM)  DFS Item

Invitrogen dTTP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dTTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

8

Invitrogen™ dATP (100 mM)  DFS Item

Invitrogen dATP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

9
Promotions Available

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents  DFS Item
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TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
PCR Method qPCR
Product Line TaqMan
10

Applied Biosystems™ TaqMan™ Microbe Detection Assay  DFS Item

Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes.

11

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits
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The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM + VIC
Quantity 100 reactions
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
12
Promotions Available

Absolute Q™ Custom FAM/VIC Genotyping dPCR Assay  DFS Item

Absolute Q™ Custom FAM/VIC Genotyping dPCR Assay

15
Promotions Available

Applied Biosystems™ TaqMan™ β-Actin Detection Reagents

TaqMan™ β-Actin Detection Reagents