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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (650)
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Invitrogen™ SuperFi™ Buffers  DFS Item

Invitrogen SuperFi Buffer is a 5X buffer solution optimized for use with Platinum SuperFi DNA Polymerase.

Quantity 4 x 1.25 mL
For Use With (Application) Hot-start PCR
3

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
4

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix  DFS Item

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.

Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Size (Final Product) 1.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
5

Applied Biosystems™ True Allele™ PCR Premix  DFS Item

Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.

6

Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase  DFS Item

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.

7

Invitrogen™ Platinum™ II Green PCR Buffer (5X)  DFS Item

Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR

8

Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)  DFS Item

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
Starting Material DNA
For Use With (Application) Hot-start PCR
9

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase  DFS Item

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq DNA Polymerase
Format One-Step RT-PCR System
Size (Final Product) Up to 4.5 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
10

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase  DFS Item

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq High Fidelity
Format One-Step RT-PCR System
Size (Final Product) Up to 10 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
11

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System  DFS Item

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
12

Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase  DFS Item

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

13

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase  DFS Item

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Shipping Condition Wet or Dry Ice
Polymerase Tsp Genotyping Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 500 bp or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR
14

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2  DFS Item

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
15

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2  DFS Item

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'