PCR Equipment and Supplies

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Invitrogen™ Second Strand cDNA Synthesis Kit  

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR  

SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit  

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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit  

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit  

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR  

The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)⁺ or total RNA.

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Invitrogen™ DNA Polymerase I  

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Invitrogen™ DNA Polymerase I/DNase I  

An optimized mixture of both enzymes for efficient nick translation of DNA

Invitrogen™ T4 DNA Polymerase  

DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity

Thermo Scientific™ DNA Polymerase I (10 U/μL)

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Thermo Scientific™ dsDNase

dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.