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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

2

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence
4

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Product Type ECL Western Blot Substrate
5

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

6

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Sensitivity Low picogram to mid-femtogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Detection Method Chemiluminescence, Fluorescence
7

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal
Detection Method Chemiluminescence
8
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Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
GREENER_CHOICE

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EDGE
Sensitivity ∼1 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
9
Promotions Available

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

Sensitivity ∼10 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Western Blot
10
Promotions Available

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

11

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

12

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

13

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots