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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

2

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

4

MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit

Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.

5

Invitrogen™ Tris-Glycine Transfer Buffer (25X)  DFS Item

Made with high-purity reagents and are strictly quality controlled

6

MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma

Ready to use blocking reagent for western blotting immunoassays.

7

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer  DFS Item

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

8

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
9

Invitrogen™ iBind Flex PLUS Solution Kit

The iBind Flex PLUS Solution Kit increases signal sensitivity, requires less antibody per run, and simplifies western blotting immunoprocessing when used with the iBind Flex Western System. The iBind Flex PLUS Solution Kit comes with two ready to use solutions, iBind Flex PLUS Solution and iBind Flex PLUS Antibody Diluent.

Product Type Flex Solution Kit
10

Invitrogen™ XCell II™ Blot Module  DFS Item

Easy transfer of proteins or nucleic acids from mini-gels to membranes

11

Thermo Scientific Chemicals Nitrocellulose stripping buffer (10X)

Form Liquid
Product Type Western Blot Stripping Buffer
Concentration 10X
12

MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

For Use With (Application) Western Blot
13

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Form Liquid
Product Line Restore
14

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Form Liquid
Product Line Restore
15

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)  DFS Item

Optimized, easy-to-use blocker and primary antibody diluent system