HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

Pricing and Availability

Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns

Retain and resolve polar analytes with highly aqueous mobile phases.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	12%
Temperature	60°C
Phase	Reversed Phase
Endcapped	Polar
pH	2 to 9
Surface Area	220 m²/g
USP Type	L1

MilliporeSigma™ Supelco™ Kromasil C8 HPLC Column, 100 Å, 5 μm Particle Size

For small molecules and peptides.

Pricing and Availability
Specifications

Pore Size	100 Å
Particle Size	5 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase
Product Line	Kromasil

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

Pricing and Availability

Thermo Scientific™ Hypersil™ MOS C8 HPLC Columns

Use as a general-purpose C8 phase for acidic and neutral analytes.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	6.5%
Stationary Phase	C8
Temperature	60°C
Phase	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	Hypersil Classical
USP Type	L7

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns

Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.

Pricing and Availability
Specifications

Pore Size	120 Å
Stationary Phase	Polar Embedded
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
Surface Area	300 m²/g
USP Type	L60

Thermo Scientific™ Hypersil GOLD™ Phenyl HPLC Columns

Use for quick analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

Pricing and Availability
Specifications

Pore Size	175 Å
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
pH	2 to 8
Surface Area	220 m²/g
USP Type	L11

Thermo Scientific™ Hypersil™ Phenyl-BDS HPLC Columns

Use as a robust, general-purpose column with aromatic selectivity for QC/QA labs.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	5%
Stationary Phase	Phenyl (Ph)
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Base-Deactivated Silica
Column Type	Reversed Phase
Surface Area	170 m²/g
Max. Pressure	5800 psi (400 bar)
USP Type	L11

Thermo Scientific™ Hypersil™ Green PAH HPLC Columns

Use for rapid analysis of polyaromatic hydrocarbons (PAHs). They feature tailored alkyl-bonded silica with high carbon loading for excellent performance.

Pricing and Availability
Specifications

Pore Size	120 Å
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Surface Area	170 m²/g
Max. Pressure	5800 psi (400 bar)

Thermo Scientific™ Syncronis™ C8 HPLC Columns

Use this C8 column for hydrophobic interaction reversed-phase separations.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	10%
Stationary Phase	C8
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	320 m²/g
USP Type	L7

Thermo Scientific™ Hypersil GOLD™ C8 HPLC Columns

Have analytes with medium hydrophobicity or need a less hydrophobic phase to obtain optimum retention? These columns provide outstanding sensitivity and excellent peak shape.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	8%
Stationary Phase	C8
Temperature	60°C
pH	2 to 9
Surface Area	220 m²/g
USP Type	L7

Thermo Scientific™ BetaBasic™ Phenyl Columns, 5μm Particle Size

BetaBasic columns are highly efficient and reproducible for small molecules, peptides and protein digests

Pricing and Availability
Specifications

Pore Size	150 Å
Particle Size	5 μm
Carbon Load	7%
Stationary Phase	Phenyl (Ph)
Phase	Reversed Phase
For Use With	LC/MS Separation
Endcapped	Yes
Packing Material	Silica
Column Type	Reversed Phase
Surface Area	200 m²/g
Particle Shape	Spherical
Film Thickness	5 μm
USP Type	L11

Thermo Scientific™ Hypersil™ ODS-2 C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes separated under difficult operating conditions.

Pricing and Availability
Specifications

Pore Size	80 Å
Carbon Load	11%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous Type A Silica
Column Type	Reversed Phase
pH	2 to 8
Surface Area	220 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	Hypersil Classical
USP Type	L1

Thermo Scientific™ BioBasic™ 4 HPLC Columns

Use this 300Å wide pore silica for analysis of larger hydrophobic proteins and peptides that bind too tightly on C8 or C18 columns.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	4%
Stationary Phase	C4
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
USP Type	L26

Thermo Scientific™ Syncronis™ Phenyl HPLC Columns

Use this high-surface-area column for aromatic compound separations.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	11%
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	320 m²/g
USP Type	L11

HPLC Columns

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Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns